摘要
目的 揭示贵州省 HIV－ 1感染毒株的亚型分布及其流行态势。方法 用巢式 PCR法扩增 HIV－ 1膜蛋白基因 (env)，并经序列分析。结果 核酸分离成功的 11份全血标本中， B亚型 7例， C、 E亚型各 2例； B亚型基因离散率为 3.21％± 1.4％，流行时间约 3年， E和 C亚型基因离散率为 1.65％和 0.98％，流行时间约 1年。结论 贵州省 HIV－ 1感染毒株亚型分布广泛，来源复杂，预示今后将以性传播途径为主，防治难度较大。
Objective To reveal the distribution of subtypes of HIV－ 1 and their epidemic status in Guizhou Province. Methods The provirus DNA of HIV was extracted from the peripheral blood mononuclear cells in 11 HIV－ 1 infected individuals, the env gene of HIV was analysed by nested PCR and DNA sequencing. Results Among eleven HIV positive blood samples, 7 were subtype B (accounting for 64％ ), 2 were subtype C (accounting for 18％ ), another 2 were subtype E (accounting for 18％ ). The genic dispersion rate of subtype B was 3.21± 1.4％ , with 3 years epidemic in Guizhou. The genic dispersion rates among subtype E or C were 1.65％ or 0.98％ , respectively, with 1 year epidemic in Guizhou. Conclusions There are various subtypes of HIV strains in Guizhou Province,transmitted from different sources, which indicates that sexual transmission will be dominant in the future, and also more difficult to control.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2000年第3期149-151,共3页
Chinese Journal of Dermatology
基金
贵州省自然科学基金
