摘要
目的 了解陕西省HIV-1毒株的流行情况和亚型特征。方法 用套式聚合酶链式反应（Nested-PCR）对6份采集于陕西省经确认为HIV-1感染者或艾滋病病人外周血淋巴细胞（PBMC）提取核酸,从6份样品中获得了HIV-1膜蛋白（ENV）基因的核酸片段进行扩增,并测定和分析了C2-V3及其邻区共312个核苷酸序列。结果6份血样中,2份为HIV-1A亚型毒株感染（SHX7、SHX8）;4份为HIV-1B’亚型（泰国B’亚型）毒株感染（SHX1、SHX5、SHX6、SHX9）。SHX1、SHX5、SHX6、SHX9彼此间基因离散率为5.62％,SHX7与来自卢旺达的U08794之间的基因离散率仅为2.41％。结论 陕西省目前存在HIV-1A、B’两种亚型的毒株,HIV-1B’亚型由邻近地区传入,HIV-1A亚型为与非洲人接触传入。
Objective To study the epidemic situation and characteristics of subtypes of HIV-1 strains prevalent in Shanxi Province. Methods Six DNA fragments of HIV-1 ENV gene were amplified by nested PCR from uncultured peripheral blood mononuclear cells(PBMCs)obtained from six HIV- 1 seropositive individuals or AIDS patients identified in Shanxi Province.The C2 - V3 region (about 450bp) was sequenced.Results There were two strains of HIV-1 subtype A and four strains of HIV-1 subtype B', and the gene divergence of B' subtype was 5.62% .The 2 strains of HIV-1 subtype A were closely related to the U08794 from Rwanda, while the 4 strains of HIV - 1 subtypes B' were closest to those of subtype B in Thailand.Conclusion The study and epidemiological data showed that strains of HIV-1 subtype A and B' were prevailing in Shanxi Province.
出处
《中国性病艾滋病防治》
2001年第3期158-160,共3页
Chinese Journal of Std & Aids Prevention and Control
