摘要
目的研究HLA-B27阳性强直性脊柱炎(AS)患者淋巴细胞含量、DKK1和骨形成蛋白-2(BMP-2)因子表达特点及临床意义。方法采用流式细胞术分别检测患者及健康者CD4+T、CD8+T、B及NK细胞百分率,并分析CD4+T/CD8+T比值,应用酶联免疫吸附测定(ELISA)分别检测患者及健康者DKK1和BMP-2因子的表达。结果与对照组相比,HLA-B27阳性AS患者总T细胞含量百分比显著下降,差异有统计学意义(P<0.05);B细胞含量百分比显著升高,差异有统计学意义(P<0.05),DKK1和BMP-2的表达均显著上升,差异有统计学意义(P<0.05)。结论免疫系统及DKK1和BMP-2异常可能是HLA-B27阳性AS发病机制之一,值得进一步深入研究。
Objective To study the content of lymphocytes,the expression of DKK1 and BMP-2 in patients with ankylosing spondylitis（AS） and HLA-B27 positive and investigate the clinical significance.Methods The percentages of CD4＋T,CD8＋T,B and NK were detected in patients and healthy people by Flow cytometry,and the ratio of CD4＋T/CD8＋T was analyzed.The expression of DKK1 and BMP 2 of patients and healthy people were detected by ELISA technique.Results Compared with the control group,the total percentage of T cells decreased significantly（P0.05）,the percentage of B cells increased significantly（P0.05）,the expression of DKK1 and BMP-2 increased significantly（P0.05） in patients with AS and HLA-B27 positive.Conclusion Dysfunction The immune system,DKK1 and BMP-2 may be involved in pathogenesis of AS with HLA-B27 positive.It is worth further research.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第36期3857-3858,3861,共3页
Chongqing medicine
