摘要
宏基因组学研究特定生物环境中全部微小生物的基因组,直接从土壤和海水以及人体胃肠道和口腔等环境中获取样品DNA,利用适宜的载体将其克隆到替代宿主细胞中构建宏基因文库,以筛选新的活性物质和新的基因;因此,利用宏基因组学技术不仅能够有效地检测口腔微生物群落结构,同时还极大地扩展了口腔微生物资源的利用空间,增加了获得新的生物活性物质和基因的机会。本文就宏基因组学的研究方法和宏基因组学口腔微生物研究领域中的应用等研究进展作一综述。
Metagenomics is the genomes of the total microbiota found in nature. Metagenome libraries were con- structed by directly extracting DNA from environmental samples, Such as soil, oceans, human gastrointestinal tract and mouth, etc., and transforming to surrogate host. The libraries were screened for novel bioactive compounds or genes. Therefore, Metagenomics can effectively detect oral microbial community structure, and also can enormously amplified the space of microbial resource utilization and enhanced the opportunity of obtain novel bioactive com- pounds. This review included the research progress of the methods of metagenomic and the application of metage- nomic study in the oral microbiota.
出处
《国际口腔医学杂志》
CAS
2013年第1期55-58,63,共5页
International Journal of Stomatology
基金
国家自然科学基金资助项目(81170954)
关键词
宏基因组学
微生物群落
遗传物质
口腔
metagenomics
microorganism structure
genetic material
oral
