摘要
目的:建立高效液相色谱法同时测定高冠平胶囊中三七、红参所含皂苷类成分含量的方法。方法:采用高效液相色谱法,色谱柱为HibarC18(250mm×4.6mm,5μm);流动相A为乙腈,B为水;流速为1.0mL.min-1;检测波长为203nm;洗脱程序:0～35min,A相为19%,B相为81%;35～85min,A相为19%→35%,B相为81%→65%;85～100min,A相为35%→19%,B相为65%→81%。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1分别在0.4104～3.2832μg、2.0884～16.7072μg、1.222～9.776μg、2.0072～16.0576μg范围内呈良好的线性关系,其回收率分别为98.92%、98.97%、99.01%、99.25%,RSD分别为1.08%、1.06%、0.85%、1.15%。结论:本方法简便、准确、重现性好,可用于高冠平胶囊的质量控制。
Objective： To establish an HPLC method for determination of Saponins of Notoginseng Radix et Rhizoma and Ginseng Radix et Rhizoma Rubra in Gaoguanping capsules. Methods：The HPLC was performed on Hibar C18 （250mm × 4. 6mm）, acetonitrile as mobile phase A ,water as mobile phase B. The flow rate was 1.0 mL · min^-1. The detection wavelength was 203 nm. Gradient elution： 0 - 35 min, A： 19%, B： 81%; 35 -85 min,A：19%→35% ,B： 81% →65% ;85 -100min, A：35%-d9%, B：65-81%. Results： The linear range of notoginsenoside RI, ginsenoside Rg1 ginsenoside Re and ginsenoside Rbl was O. 4104 -3.2832μg, 2.0884 - 16. 7072μg, 1. 222 -9. 776μg, 2. 0072 - 16. 0576μg with good correlation. The average recovery was 98.92%, 98.97%, 99. 01%, 99. 25%, RSD was1. 08% ,, 1.06%, 0. 85%, 1.15%. Conclusion： The method is simple, accurate and reproduciable. It can be used for quality control of notoginsenoside R1 , ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Gaoguanping capsules.
出处
《世界中医药》
CAS
2012年第6期557-559,共3页
World Chinese Medicine
基金
2009年度陕西省科学技术研究发展计划项目(编号:2009k19-03)
