摘要
目的从分子层面了解癌痛消方抗肿瘤的主要作用机制,明确不同功效药物组在抗肿瘤中所扮演的角色,从而深入阐释"癌毒"论的客观实质性。方法采用Walker-256瘤株进行Wistar大鼠的移植性肝癌的造模,造模成功后随机分为5组,即全方组、理气活血(拆方Ⅰ)组、清热解毒(拆方Ⅱ)组、扶正培元(拆方Ⅲ)组、模型组,每组10只,分别给予相应药物治疗14天,最后处死大鼠,并取出瘤块,分别应用流式细胞仪Annexin V/PI法检测肿瘤细胞凋亡率和细胞周期、免疫组化法和实时荧光定量RT-PCR法检测瘤块中Bcl-2、Survivin蛋白和mRNA的表达情况。结果 (1)全方组能明显促进肝癌细胞凋亡;并能影响肝癌细胞周期,使大部分细胞停滞于G0/G1期,有效阻止肝癌细胞增殖。拆方Ⅱ组与全方组疗效一致,差异无统计学意义(P>0.05);拆方Ⅲ组有效,但其效果不如前两者,差异有统计学意义(P<0.01);(2)癌痛消方能明显下调肝癌细胞中Survivin和Bcl-2蛋白和mRNA的表达;清热解毒药物在下调肝癌细胞中Survivin和Bcl-2蛋白和mRNA的表达效果显著,与癌痛消方功效相近(P>0.05),扶正培元药物疗效不及前两者,差异有统计学意义(P<0.01),活血化瘀药物一定程度上上调Survivin和Bcl-2蛋白和mRNA的表达。结论 (1)癌痛消方能明显通过下调肝癌细胞中Survivin和Bcl-2蛋白和mRNA的表达,致使肝癌细胞凋亡比值升高,肝癌细胞增殖受到抑制;(2)清热解毒药物在癌痛消方中起到了最为重要的作用,湿热毒邪是肿瘤发病中最根本的致病因素,正气亏虚也是肝癌起病的条件之一,痰、瘀等病理产物作用机制不明,有待进一步研究。
Objective To study the main mechanisms of Aitongxiao Recipe （ATXR） for anti-tumor at the molecular level, and to clarify different efficient drugs＇ roles in anti-tumor, thus in-depth explaining the objectivity and substance of ＂cancer toxic＂ theory. Methods Walker-256 tumor strain was used for Wistar rat transplanted liver cancer modeling. After successful modeling rats were randomly divided into 5 groups, i. e., the ATXP group, the qi regulating and blood circulating group （as the assembled I group）, the heat clearing and detoxifi- cation group （as the assembled II group）, the body resistance strengthening and cultivating group （as the assembled III group）, and the model group, 10 in each group. Corresponding medication was given to rats in each group for 14 successive days. Finally rats were sacrificed and the tumor mass was taken out. The apoptosis rate and the cell cycle of tumor cells were detected by flow cytometry Annexin V/PI. The protein and mRNA expressions of Bcl-2 and survivin were detected using immunohistochemistry and real-time fluorescent quantitative PCR. Results （1） The apoptosis of hepatoma carcinoma cells could be obviously promoted in the ATXP group. The cell cycle could also be affected, making major cells arrest at G0/G1 phase. The proliferation of hepatoma carcinoma cells was effectively prevented. The efficacy in the assembled II group was in line with that in the ATXP group with no statistical difference （ P 〉0. 05）. It was also effective in the assembled III group, but its efficacy was not as good as that in the former two groups, showing statistical difference （P〈0.01）. （2） ATXP could ob- viously down-regulate the protein and mRNA expressions of Bcl- 2 and survivin in hepatoma carcinoma cells.Drugs for heat clearing and detoxification showed significant effects on down-regulating the protein and mRNA expressions of Bcl- 2 and survivin in hepatoma carcinoma cells. Their effects were similar to that of ATXP （ P 〉 0.05）. The effects of drugs
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2012年第12期1652-1657,共6页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.30960475)
关键词
癌痛消方
湿热毒邪
肝癌细胞
Aitongxiao Recipe
damp-heat insidious pathogen
hepatoma carcinoma cell
