摘要
目的:研究大鼠坐骨神经缺损后损伤近端神经组织中胞外基质分子TNC(tenascin-C)的表达变化及初步功能分析。方法:采用冰冻切片和免疫荧光染色法,检测神经缺损0d(对照组),4d(实验组)损伤近端神经组织TNC的表达变化及表达位置;并利用体外共培养施万细胞/成纤维细胞及细胞划痕实验初步研究TNC的作用。结果:免疫荧光染色发现TNC在损伤后4d处于高表达状态,并在神经外膜处与波形蛋白(Vimentin)共定位状况良好;体外细胞共培养实验发现下调成纤维细胞中的TNC可以降低与之共培养的施万细胞的迁移速度。结论:在神经损伤后成纤维细胞分泌TNC并比对照组表达大幅上调,且体外实验发现TNC可促进施万细胞的迁移速度。
Objective： To investigate the expression and function of TNC （tenascin-C） in the proximal nerve stump following sciatic nerve transection. Methods：The expression level and localization of TNC in the proximal nerve stump were examined by frozen section and immunofluorescent staining on 0d and 4d after sciatic nerve transection, and the function of TNC was examined by fibroblasts/Schwann cells co-culture and cell scratch wound assay.Results：The expression of TNC in the proximal nerve stump up-regulated on 4d after sciatic nerve transection and co-localized with Vimentin in epineurium; TNC could promote migration of Schwann cells in vitro co-culture assay.Conclusion：This study shows that TNC secreted by fibroblasts up-regulated after sciatic nerve transection and TNC could promote migration of Schwann cells in vitro.
出处
《交通医学》
2012年第5期401-403,406,共4页
Medical Journal of Communications
基金
国家863计划项目(2012AA020502)
国家自然科学基金资助项目(81130080)
关键词
坐骨神经缺损
胞外基质
TNC
成纤维细胞
施万细胞
逆转录聚合酶联反应
免疫荧光染色法
共培养
迁移
大鼠
proximal nerve stump
extracellular matrix
tenascin-C
fibroblasts
Schwann cells
reverse transcriptatepolymerase chain reaction
immunofluorescent staining
co-culture
migration
rat
