摘要
对一株产纤溶酶的海洋枯草芽孢杆菌LJ-22的固、液体发酵条件分别进行了优化,确定该菌株的最佳液体发酵条件为温度31℃,初始pH7.0,转速180r/min,接种量4%,培养时间72h,最佳固体发酵条件为温度34℃,初始pH7.0,接种量10%,装样量10g,静置培养时间72h。在上述条件下,菌株LJ-22的液体发酵产纤溶酶酶活为(830±12.5)IU/mL,是优化前的1.69倍,固体发酵产纤溶酶酶活为(4300±12.8)IU/g,是优化前的1.56倍。通过比较固、液体发酵条件及产酶酶活,得出该菌株更适合液体发酵,便于大规模工业生产。
The marine Bacillus subtils L J-22 which produces fibrinolytic enzyme was optimized its solid and liquid fermentation conditions ,determine the optimal liquid fermentation conditions were as follows ：temperature 31X：;,initial pH7.0,inoculum 4% ,the rotation speed 180r/min for 72h. The optimal solid fermentation conditions were as follows temperature 34℃, initial pH7.0, inoculums 10%, sample loading volume 10g, fermentation cultivate for 72h.Under those conditions,the liquid fermentation produce the activity of fibrinolytic enzyme reached （830±12.5）lU/mL,which was 1.69 folds as high as that of the initial strain,the solid fermentation produce the activity of fibrinolytic enzyme reached （4300±12.8）lU/g,which was 1.56 folds as high as that of the initial strain. Through comparing the solid fermentation conditions and the activity of fibrinolytic enzyme with the liquid fermentation＇s,it was found that the marine Bacillus subtils LJ-22 was more suite for liquid fermentation ,meanwhile was facilitate large-scale industrial production.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第24期249-252,共4页
Science and Technology of Food Industry
基金
国家自然科学基金资助项目(21062001)
关键词
纤溶酶
海洋枯草芽孢杆菌
液体发酵
固体发酵
fibrinolytic enzyme
marine Bacillus subtilis
liquid fermentation
solid fermentation
