摘要
研究了石榴皮中总生物碱的提取工艺并对其含量进行测定。以酸性染料比色法为分析手段,通过正交试验,探讨了影响总生物碱提取率因素的范围和最佳组合。石榴皮中总生物碱的最佳提取工艺条件为:硫酸铵8.0g、丙醇用量20mL、醇水比为0.8∶1的双水相体系、超声时间60min。在此条件下,石榴皮中总生物碱的平均提取率为0.559mg/g,平均回收率为100.8%。
The total alkaloids were extracted from Pomegranate Peel and determined with acid dye colorimtric method. By orthogonal test, the highest extraction yield was obtained when the amount of ammonium sulfate was 8.0 g,the volume propanol was 20 mL,the alcohol/water ratio of the two-phase extraction system was 0.8 and ultrasonic extraction time was 60 rain. Under the optimized conditions, the extraction rate was 0. 559 mg/g with an average recovery of 100.8%.
出处
《分析科学学报》
CAS
CSCD
北大核心
2012年第6期883-885,共3页
Journal of Analytical Science
关键词
石榴皮
总生物碱
酸性染料比色法
超声波
双水相
Pomegranate peel
Total alkaloids
Acid dye colorimetry
Aqueous two-phase
