摘要
目的:研究热习服对高温力竭运动大鼠血清Na+、K+、抗利尿激素(ADH)、醛固酮(ALD)、心钠素(ADH)含量和下丘脑AVP合成的影响。方法:24只雄性Wistar大鼠随机分为空白对照组(A组)、高温力竭组(B组)、热习服后高温力竭组(C组)。常温环境温度25℃,湿度50%;高温环境温度38℃,湿度50%。A组不进行任何处理,C组每天在高温环境下以18 m/min、坡度0在跑台训练14天,第15天B组和C组在高温环境下以20 m/min、坡度0运动到力竭。实验前后各组大鼠称量体重并测肛温,记录B、C组大鼠力竭运动时间。后麻醉取血和下丘脑,测定血清Na+、K+、ANP、ADH、ALD含量,RT-PCR测定下丘脑ADH mRNA表达。结果:(1)C组大鼠力竭运动时间(75.20±15.39 min)明显长于B组大鼠(27.67±4.80 min,P<0.01)。(2)C组大鼠体重丢失%明显高于B组(P<0.01);C组大鼠血浆渗透压(316.50±3.63 mmol/L)明显高于B组(304.75±5.90 mmol/L,P<0.01)。(3)B、C组大鼠血清ADH浓度显著高于A组(P<0.05),同时C组显著低于B组(P<0.05);B组和C组大鼠血清ALD浓度高于A组(P<0.01);B组和C组大鼠血清ANP浓度低于A组(P<0.05)。(4)B组大鼠下丘脑ADH mRNA表达(1.51±0.18)比A组(1.04±0.18)升高(P<0.01),C组表达(1.23±0.38)也高于A组(P<0.05)。结论:热习服明显提高大鼠在高温环境下的运动能力。热习服后,血液抗利尿激素浓度降低,下丘脑渗透压感受器"调定点"上移,血浆渗透压变化与下丘脑分泌ADH不一致。
Objective The purpose of this paper was to observe the effect of heat acclimatization on serum Na＋, K＋, antidiuretic hormone （ADH）, and atrial natriuretic peptide （ANP） after prolonged exercise in a warm environment. Methods 24 male Wistar rats were randomly divided into 3 groups：control group （A） ,prolonged exercise in warm environment group （B） and prolonged exercise in warm environment af- ter heat acclimatization group（C）. The temperature and humidity of normal environment were set at 25℃ and 50% ,respectively. The warm environment was created in a chamber with 38℃ of temperature and 50% of humidity. Rats in group C underwent exhaustive treadmill training at the speed of 18m/rain andslope of 0° for 14 days in warm environment. Then,rats in groups B and C carried on treadmill training at the speed of 20m/min and slope of 0° in warm environment till exhaustion. Body weight and rectal temperature of the rats were measured before and after the experiment, and exhaustive exercise duration of groups B and C was recorded. Immediately after exhaustion, serum Na＋, K＋, ANP, aldosterone （ALD）, ADH in hypothalamus were tested. Results （2）The exhaustive exercise duration of group C （75.20±15.39 min） was significantly longer than group B （27.67±4.80 min）（P 〈 0.01）. （2）The weight loss in % of group C was greater than that of group B（P 〈 0.01）;plasma osmotic pressure of group C（326.50 ±3.63 mmol/L） was significantly higher than that in group B （304.75±5.90 mmol/L）（P 〈 0.02）. （3）After ex- haustive exercise in warm environment,the concentration of serum ADH in groups B and C was higher than that in group A（P 〈 0.05） ,and in group C was lower than that in group B（P 〈 0.05） ;The concen- tration of serum ALD of groups B and C was higher than that of group A （P 〈 0.02 ）, The concentration of serum ANP of group B and C was lower than that of group A （P 〈 0.05）.（4）The expression of ADH mRNA in hypothalamus of group B
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2012年第11期983-987,993,共6页
Chinese Journal of Sports Medicine
基金
国家体育总局体育科学研究所基本科研业务课题(08-13)
关键词
高温
力竭性运动
抗利尿激素
心钠素
下丘脑
heat acclimatization
warm environment
exhaustive exercise
ADH
ANP
hypothalamus
