摘要
目的使用小干扰RNA（siRNA）干扰人前列腺癌LNCaP细胞肝再生磷酸酶-3（PRL-3）基因的表达，观察PRL-3基因沉默后对LNCaP细胞生长增殖及侵袭力的影响。方法实验对象包括携带可稳定抑制PRL-3基因表达siRNA慢病毒的LNCaP细胞组（实验组），携带对任何基因无干扰作用siRNA慢病毒的LNCaP细胞组（空转组），同时建立未进行处理的普通LNCaP细胞组（对照组），用噻唑蓝（MTT）比色法检测3组细胞生长增殖，Transwell法观察3组细胞的侵袭能力。结果与空转组及对照组比较，siRNA干扰LNCaP细胞后，实验组PRL-3mRNA和蛋白水平都明显降低（P〈0．05），实验组穿过人工基底膜细胞数明显小于空转组及对照组（P〈0．01），但是对细胞生长影响不大。结论降低PRL-3基因的表达对LNCaP细胞生长抑制作用不明显，但能明显抑制LNCaP细胞的侵袭能力。
Objective To investigate influence of phosphatase of regenerating liver-3 （PRL-3） gene silencing with small interference RNA （siRNA） on biological behaviors of human prostate cancer LNCaP cells, including proliferation and invasion. Methods The method of silencing PRL-3 was estab- lished by lentivirus-mediated RNAi. The cells were divided into 3 groups. In experimental group, the expres- sion of PRL-3 in LNCaP cells was stably blocked by lentivirus-mediated RNAi. In negative control group, the cells were transfeeted with lentivirus-mediated control RNAi （without any interference to PRL-3 ）. The nor- mal LNCaP cells served as blank control group. Methyl thiazol tetrazolium （ MT1~） assay was used to meas- ure the proliferation of LNCaP cells, and Transwell assay was used to observe invasion potency. Results The experimental group showed significantly lower levels of PRL-3 mRNA and protein than those in negative control and blank control groups after transfection （ P 〈 0. 05 ）. The invasion potency of LNCaP cells was significantly suppressed after transfeetion （ P 〈 0. 05 ） , but there was no significant difference among the three groups on proliferation. Conclusion Down-regulation of PRL-3 expression can weaken invasion potency of LNCaP cells, but it had no effect on proliferation.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第12期2370-2372,共3页
Chinese Journal of Experimental Surgery
基金
浙江省中医药管理局资助项目（2011ZB092）
关键词
前列腺癌
肝再生磷酸酶-3
RNA干扰
Prostate cancer
Phosphatase of regenerating liver-3
RNA interference
