摘要
目的:观察哮喘患者外周血CD4+CD25+Foxp3+、CD4+IL-17+细胞的百分比和Foxp3、白介素-17(IL-17)、RORγt mRNA的表达水平的变化与哮喘发病的关系。方法:分别取23例哮喘缓解期患者、25例哮喘急性发作期患者和20例健康人(对照组)的外周血单个核细胞(PBMC),免疫磁珠阴选CD4+T细胞,采用Foxp3、IL-17胞内染色的方法,通过流式细胞术检测CD4+CD25+Foxp3+以及CD4+IL-17+细胞的百分比,应用RT-PCR检测Foxp3、IL-17、RORγtmRNA水平。结果:免疫磁珠阴选CD4+T细胞纯度达90%以上。急性发作组患者外周血CD4+CD25+Foxp3+细胞百分比以及Foxp3 mRNA水平较对照组和缓解组明显降低(P<0.01),而缓解组和对照组比较,差异无统计学意义(P>0.05);急性发作组CD4+IL-17+细胞比例以及IL-17、RORγt mRNA表达较对照组和缓解组明显增高(P<0.01),而缓解组亦明显高于对照组(P<0.05)。结论:Treg/Th17失衡可能和哮喘发病密切相关。
Objective：To study the percentage of CD4＋CD25＋Foxp3＋,CD4＋IL-17＋ cells and the expression Foxp3,IL-17,RORγt mRNA in peripheral blood from patients with asthma.Methods：The specimens of peripheral blood mononuclear cell（PBMC） were collected from 23 patients of asthma in remission stage,25 patients of acute paroxysmal asthma and 20 healthy persons（control group）.Intracellular flow cytometry detection of the percentage of CD4＋CD25＋Foxp3＋ cells and CD4＋IL-17＋ cells was established after isolation of untouched human CD4＋T cells from PBMC.The expression level of Foxp3,IL-17,RORγt mRNA in CD4＋T cells was determined by real-time fluorescence quantitative RT-PCR.Results：Immunomagnetic bead shade selected CD4＋T cells reached a purity of more than 90%.The percentage of CD4＋CD25＋Foxp3＋ cells and the expression level of Foxp3 mRNA of the acute paroxysmal group was significantly lower than that of the control group and remission group（P0.01）,and the comparison of the control group and remission group didn＇t have significant deviation（P0.05）.The percentage of CD4＋IL-17＋ cells and the expression level of IL-17、RORγt mRNA of the acute paroxysmal group was significantly higher than those of the control group and remission group（P0.01）,and the expression level of IL-17、RORγt mRNA in the remission group was also significantly higher than the control group（P0.05）.Conclusion：The percentage of CD4＋CD25＋Foxp3＋cells and the expression of Foxp3 mRNA in peripheral blood from patients with asthma decreased significantly.The percentage of CD4＋IL-17＋cells and the expression of IL-17、RORγt mRNA in peripheral blood from patients with athma increased significantly.The imbalance of Treg/Th17 perhaps correlated with the acute episode of asthma.
出处
《江苏大学学报（医学版）》
CAS
2012年第6期530-533,共4页
Journal of Jiangsu University：Medicine Edition
基金
江苏大学临床医学科技发展基金资助项目(JLY2010121)
