摘要
为了明确来自湖北武穴、孝感、随州3个不同地区的转cry1Ab/cry1Ac、cry2A、cry1C基因明恢63(分别命名为TT51、T2A-1和T1C-19)对赤拟谷盗Tribolium castaneumHerbst生长发育的影响,在室内以不同的转Bt基因稻谷继代饲养赤拟谷盗6代,结果表明:各处理赤拟谷盗的卵期3～4d,卵孵化率85%～100%,幼虫期22～27d,化蛹率85%～97%,蛹期6～7d,百蛹重0.25～0.31g,性比0.8～1.4,羽化率82%～97%,产卵前期5～7d。不同抗虫转基因水稻对赤拟谷盗各发育历期及生命表参数没有显著的影响,同一转基因事件,没有因为种植地区的不同造成对赤拟谷盗生长发育的差异。3种Bt蛋白在继代饲养的赤拟谷盗幼虫体内检测均呈阳性反应,但积累量很小,继代饲养后,没有发现在赤拟谷盗体内明显的累积。
The effect of the transgenic Bt rice Shanyou 63 with crylAb/crylAc, cry2A and crylC （TTS1, T2A-1 and T1 C- 19） on the growth and development of Tribolium castaneum Herbst was investigated using rice from three different areas （Wuxue, Xiaogan and Suizhou in Hubei Province）. The results show that the duration of the egg, larval, pupal and preoviposition stages were 3 to 4 days, 22 to 27 days, 6 to 7 days and 5 to 7 days, respectively. Hatchability, pupation rate and emergence rate were 85% to 100% , 85% to 97% and 82% to 97% , respectively. The weight of 100 pupae was 0. 25 to 0. 31 g and the sex ratio was 0.8 to 1.4. No significant differences were found among the different rice varieties. All three Bt proteins could be detected in T. castaneum larvae, but the accumulated amount was very small. There was no significant accumulative effect despite larvae being reared on these strains.
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2012年第6期1525-1534,共10页
Chinese Journal of Applied Entomology
基金
国家重大科技专项(2011ZX08001-001
2008ZX08001-001)
江西省自然科学基金(20122BAB204025)
关键词
转BT基因水稻
赤拟谷盗
生长发育
安全性评价
transgenic Bt rice, Tribolium castaneum, growth and development, safety evaluation
