摘要
目的:为了探讨高尔基体蛋白73(Golgi protein73,GP73)和甲胎蛋白(AFP)联检在原发性肝癌(primary hepatic carcinoma,PHC)诊断中的临床应用。方法:采集57例PHC患者、30例良性肝胆疾病患者(胆结石和肝炎)和33例正常对照组血清。酶联免疫吸附试验(ELISA)测定GP73、时间分辨荧光免疫分析(time resolved fluorescent immunoassay,TrFIA)测定AFP。计算GP73和AFP诊断PHC的敏感性、特异性、准确度以及阴、阳性预测值,并应用Logistic回归和ROC曲线对各指标进行分析。结果:57例PHC患者血清GP73和AFP水平均明显高于30例良性肝胆疾病组与33例正常对照组,差异具有统计学意义(P均<0.05)。良性肝胆疾病组血清GP73水平高于正常对照组(P<0.05)。GP73和AFP联检,其敏感性为92.98%、特异性为88.89%、准确度为90.83%;阳、阴性预测值分别为88.33%和90.32%,与单项指标相比差异均有统计学意义(P均<0.05)。GP73、AFP的曲线下面积(area under the ROC cure,AUC)分别为0.900、0.802;两项联检AUC达0.929,>各单项,差异有统计学意义(P<0.05)。结论:血清GP73和AFP是PHC的肿瘤标志物,GP73诊断PHC的价值明显高于AFP。两项联检优于单一的肿瘤标志物,并可提高诊断PHC的正确性。
Objective In order to explore the clinical application of diagnosis through determining Golgi protein 73（GP73） combined AFP levels in patients with primary hepatic carcinoma（PHC）.Methods The GP73（by ELISA）and AFP（by time resolved fluorescent immunoassay,TrFIA）levels were determined in 57 patients with PHC,30 patients with benign hepatobiliary disease（chololithiasis and hepatitis） as well as 33 normal controls,also calculating sensitivity,specificity,accuracy and predictive value of positive and negative for PHC diagnosis,and analyze the indices by application Logistic regression and receiver operator characteristic（ROC）curve.Results The serum GP73 and AFP levels in 57 patients with PHC were significantly higher than those in patients with hepatobiliary diseases and normal controls（P all0.01）.The serum GP73 level in patients with hepatobiliary diseases distinctly higher than the normal controls（P0.05）.In combined determination of GP73 and AFP,the sensitivity,specificity and accuracy were 92.98%,88.89% and 90.83% respectively,predictive value of positive and negative were 88.33% and 90.32% discretely.These data were markedly higher than those in single marker（GP73 and AFP,P all0.05）.The area under the ROC curve（AUC）level of GP73 and AFP were 0.900 and 0.802,the AUC level of combined determination of GP73 and AFP was 0.929 obviously higher than that of single marker（GP73 and AFP,P all0.05）.Conclusion The serum GP73 and AFP were tumor markers of PHC.The diagnosis value of GP73 was evidently higher AFP for PHC and combined determination expressed superior to single marker（GP73 and AFP）so that enhance the degree of precision for PHC diagnosis.
出处
《放射免疫学杂志》
CAS
2012年第6期673-675,共3页
Journal of Radioimmanology
关键词
高尔基体蛋白73
甲胎蛋白
原发性肝癌
联合测定
Golgi protein 73（GP73）
alpha-fetoprotein（AFP）
primary hepatic carcinoma（PHC）
combined determination
