摘要
对两面针茎尖的诱导产生完整再生植株的一系列过程进行了研究。目的:建立适用于两面针组培快繁的技术体系,为工厂化生产和种质资源保护提供依据和手段。方法:剪取两面针不同组织器官作为外植体,通过不同外植体处理方法对比,接种于不同配方的培养基中进行比对试验,从中筛选出最佳的实验方案。结果:用幼苗茎尖作为外植体接种于MS+6-BA3.0 mg/L+NAA0.3mg/L的培养基中能较好地诱导出芽,并成功地完成再生植株的增殖和生根培养。
Our research focuses on how to culture entire regenerated plantlets from induced meristems of Zanthoxylum nitidum. Objective： to establish a technique system of tissue culture and rapid propagation ofZanthoxylum nitidum, and further provide advice for industrial production and protection of germplasm resources. Methods： with different tissue or organs of Zanthoxylum nitidu- mas explants cultured in different growth media for comparison experiments to select optimum ex- perimental scheme. Results： using meristems of young plants as explants cultured in MS＋6- BA3.0mg/L ＋ NAA0.3mg/L medium is the best way for inducing meristems, and it successfully achieved the growth and rooting culture of regenerated plantlets.
出处
《农业研究与应用》
2012年第6期16-20,共5页
Agricultural Research and Application
关键词
两面针
外植体
茎尖
组织培养
再生植株
Zanthoxylum nitidura
explants
meristems
tissue culture
regenerated plantlets.
