摘要
目的:研究电针足三里(ST36)对大鼠腹腔粘连组织微血管生成的影响,并探讨其作用机制与胆碱能抗炎通路的关系。方法:将48只雄性Wistar大鼠随机分为6组:A组(模型组)、B组(电针足三里)、C组(迷走神经切断组)、D组(迷走神经切断+电针足三里组)、E组(α-银环蛇毒素组)和F组(α-银环蛇毒素+电针足三里组),每组8只。参考Chiang方法制作大鼠腹腔粘连模型。B组、D组、F组给予连续电针双侧足三里穴3 d,每次持续1 h;C组和D组大鼠麻醉后,在无菌条件下切断双侧腹腔迷走神经干;E组和F组大鼠术毕即腹腔注射α银环蛇毒素。各组大鼠于术后第10天处死,剪取盲肠粘连组织切片行HE染色,在奥林巴斯显微镜下观察、拍照。每张切片随机测定5个视野,计算视野内微血管数,取均值作为结果。结果:术后第10天,各组大鼠盲肠组织均出现了不同程度的增厚、挛缩,与周围肠管及肠系膜之间均形成了粗细不等的粘连带。显微镜下,各组盲肠粘连组织均可见增生的微血管,和B组相比,其余各组微血管增生程度较重(P<0.01)。B组盲肠浆膜层微血管数量(20.38±5.40)明显低于A组(45.43±6.27)(P<0.01);F组与D组盲肠粘连组织微血管数量较B组明显增多(P<0.01),二者与A组比较无明显差异(P>0.05)。结论:电针足三里对腹腔粘连大鼠粘连组织微血管生成具有明显的抑制作用,从而起到干预大鼠腹腔粘连形成的作用,其机制可能与兴奋胆碱能抗炎通路有关。
Objective： To investigate the effects of electro,acupuncturing Zusanli aeupoints （ST36） on the angiogenesis of intra-abdominal adhesion tissues, and to find out the relationship between the protective effect of EA and the cholinergic anti-inflammatory pathway. Methods ：48 male Wistar rats were randomly divided into 6 groups with each of 8 ：group A （ the model group） ,group B （ the group of electro-acupuncturing Zusanli acupoints）, group C （the vagotomy group）, group D （the group of electro-acupuncturing Zusanli acupoints and vagotomy）, group E （（x-bungatotoxin group）, group F （the group of α-bungatotoxi and electro-acupuncturing Zusanli acupoints）. Model of abdominal adhesion was produced according to Chiang＇s operative way, in which the appendix of cecum was scraped and a sili- cone path was fixed on the lateral abdominal wall;Group B, D and F were given three days electro-acupuncture at Zusanli acupoints with once of one hour; Group C and D were cut off Vagus nerve cord in bilateral abdominal cavity under the condition of aseptic condition af- ter anesthesia;Group E and F were injected α-bungatotoxin into abdominal cavity after surgery. Rats in every group were put to death on the lOth day of surgery, then cecum adhesion tissue section were taken and made HE colouration, and were observed and photographed under Olympus. Five fields of view were randomly elected and determined for each section, then calculating number of capillaries in view fields,taking mean value as the last results. Results：After the lOth day of surgery, cecum tissues of all rats at every group presented in- crassation and contracture at varying degrees, and formed thick or thin adhesive band with round intestinal canal and mesentery. Micro- scopically, cecum tissues of every group showed hyperplastic capillaries, and comparing with group B, hyperplastic degree of capillaries were significantly higher in other five groups （ P 〈 0.01 ）. Number of capillaries at cecum serosa layer in group B was o
出处
《中医学报》
CAS
2012年第12期1609-1611,共3页
Acta Chinese Medicine
关键词
腹腔粘连
电针
足三里
微血管
胆碱能抗炎通路
大鼠
Abdominal adhesions
electro-acupuncture
Zusanli acupoint
capillaries
cholinergic anti-inflammatory pathway
rat
