摘要
目的研究沉默信息调节因子1(SIRT1)在结直肠癌中的表达,分析SIRT1与结直肠癌患者相关临床病理指标及多药耐药基因P-糖蛋白(P-gp)、谷胱甘肽转移酶π(GST-π)、DNA拓扑异构酶Ⅱ(TOPO-Ⅱ)之间的关系。方法应用免疫组化方法检测60例结直肠癌组织和24例正常结直肠黏膜中SIRT1的表达情况。结果 60例结直肠癌中,SIRT1在结直肠癌中的阳性表达率高于正常结直肠黏膜(P<0.05),60例结直肠癌中,浸润深度T3～T4者比T1～T2者SIRT1阳性表达率高,86.8%vs 42.9%(P<0.05);有淋巴结转移的比无转移的SIRT1阳性表达率高,93.3%vs 70.0%(P<0.05);pTNM分期Ⅱ、Ⅲ、Ⅳ期比Ⅰ期SIRT1阳性表达率高85.7%、83.3%、90.9%vs20.0%(P<0.05);SIRT1表达与结直肠癌浸润深度(r=0.365,P<0.05)、淋巴结转移(r=0.302,P<0.05)、pTNM分期(r=0.292,P<0.01)、p53蛋白的表达(r=0.335,P<0.05)、P-gp的表达(r=0.271,P<0.05)均呈正相关。结论 SIRT1在结直肠癌组织中高表达,且与肿瘤浸润深度、淋巴结转移、临床分期、多药耐药有关,可能参与了结直肠癌的发生发展及耐药,有可能成为结直肠癌恶性程度及预后评估的生物学指标之一。
Objective To investigate the expression of silent information regulator type 1 （SIRT1） and its correlation with clinicopathologic features with the expression of the drug-resistance associated gene P-gp,GST-n and DNA TOPO- ]] in colorectal cancer（CRC）. Methods The expression of SIRT1 in 60 colorectal cancer and 24 samples of corresponding normal colorectal tissues was investigated in the method of immunohistochemistry. Results In 60 colorectal cancer tissues,the positive rate of SIRT1 was significantly higher than that of normal colorectal tissues in tumor invasion T3-T4 compared with T1-T2,86.8% vs 42.9% （ P 〈0.05） ;in the lymph node metastasis as compared with that without lymph node metastasis,93.3 % vs 70.0% （ P 〈0.05） ;in the tumor stage II , III , IV compared with tumor stage I ,85.7% ,83.3% ,90.9% vs 20.0%（ P 〈0.05）. The expression of SIRT1 was positively correlated with tumor invasion（ r =0. 365, P d0.05）, the lymph node metastasis（ r =0. 302, P 〈0.05） ,pTNM tumor stage（ r =0. 292, P〈0.01）,p53（r=0.335, P〈0.05） and P-gp（ r =0. 271, P〈0.05） in colorectal cancer. Conclusion SIRT1 was up-regulated in colorectal cancer and significantly correlated with tumor invasion,lymph node metastasis, pTNM tumor stage, p53 and P-gp. SIRT1 may involve in the development of colorectal cancer and drug resistance, and may be a novel biological parameter to evaluate the malignant degree of colorectal carcinoma and to predict prognosis of colorectal cancer. The expression of SIRT1 may be a significant prognostic indicator for colorectal cancer.
出处
《临床荟萃》
CAS
2012年第24期2145-2148,F0002,共5页
Clinical Focus
关键词
结直肠肿瘤
多药耐药相关蛋白质类
谷胱甘肽转移酶
细胞凋亡
免疫组织化学
colorectal neoplasms
multldrug reslstance-associated protelns
glutathlone transferase
apoptosis
immumohistocheznistry
silent information regulator type 1
