摘要
目的研究沉默信息调节因子1（SIRT1）在合并2型糖尿病乳腺癌中的表达，探讨SIRT1与p53之间的相关性，分析在乳腺癌合并糖尿病组织与乳腺癌非糖尿病组织血脂之间的差异，并对其与2型糖尿病的关系及临床价值做出评价。方法应用免疫组化染色法对30例合并2型糖尿病的乳腺癌组织与30例乳腺癌非糖尿病组织中SIRT1和p53的表达进行检测，分析二者的相关性。结果①在合并糖尿病的乳腺癌组织中SIRT1阳性表达率明显低于非糖尿病乳腺癌组织（P〈0．05）；②SIRT1阳性组P53蛋白表达水平明显高于SIRT1阴性组（P〈0．05），乳腺癌合并糖尿病组织中SIRT1表达与p53表达呈显著正相关（P〈0．05）；③乳腺癌合并糖尿病组织中BMI以及甘油三脂明显高于非糖尿病乳腺癌组织（P〈0．05），高密度脂蛋白低于非糖尿病组织（P=0．05），而胆固醇以及低密度脂蛋白差异无统计学意义（P〉0．05）。结论SIRT1在乳腺癌合并糖尿病组织中存在过表达，但阳性表达率低于非糖尿病乳腺癌组织，提示其可能与糖尿病病程进展有关；SIRT1表达与p53表达呈显著正相关；此外SIRT1还可参与血脂的调节过程，因此它可能成为糖尿病以及乳腺癌的新的生物学指标。
Objective To study the expression of SIRT1 in breast cancer with diabetes mellitus,and analyze the correlation between SIRT1 and p53, and analyze blood lipid differences in breast cancer tissue with- out diabetes mellitus and breast cancer tissue with diabetes mellitus, and to research its relation with type 2 diabetes mellitus and assesse the clinical value. Methods Immunohistochemistry was used to examine the expressions of SIRT1 and p53 in 30 breast cancer patients with diabetes mellitus and 30 breast cancer patients without diabetic mellitus, and their correlation was analyzed. Results The positive rate of SIRT1 in breast cancer tissue with diabetes mellitus was significantly lower than that in breast cancer tissue without diabetic mellitus（ P 〈0.05 ）. In SIRT1 positive tissue, the expression level of p53 was significantly higher than that in SIRT1 negative tissue（ P 〈 0.05 ）. The expression of SIRT1 was significantly positively related with p53 expression in breast cancer tissue with diabetes （P 〈 0.05 ）. BMI and TG in breast cancer group with diabetes mellitus were significantly higher than those in breast cancer group without diabetic mellitus （ P 〈 0.05 ）, but HDL was lower（ P = 0.05 ）. However, CHO and LDL had no significant differences in both groups（ P 〉0.05 ）. Conclu- sions SIRT1 is up-regulated in breast cancer, but the positive rate of SIRT1 in breast cancer tissue with dia- betes mellitus is significantly lower than that in breast cancer tissue without diabetic, and is associated with the progression of diabetes mellitus. SIRT1 protein is significantly positively correlated to p53 expression and it may be involved in the adjustment process of blood lipids, SIRT1 might be a new biological target in diabetes and breast cancer.
出处
《国际肿瘤学杂志》
CAS
2011年第11期864-867,共4页
Journal of International Oncology
