摘要
目的研究贵州布依族人群载脂蛋白E基因多态性分布特征,分析apoE基因多态性对脂质代谢的影响。方法应用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)对贵州省黔南州201例布依族和217例汉族人群血样品进行apoE基因分型,并研究等位基因频率特征与血脂的关系。结果 201例布依族和217例汉族apoε2、ε3、ε4等位基因频率分别为:6.71%、82.34%、10.95%;9.22%、84.10%、6.68%,二者差异无统计学意义(P>0.05)。与其他种族、民族人群比较,布依族ε2、ε3、ε4等位基因频率处于中等水平。布、汉两民族apoE不同基因型亚组血脂、载脂蛋白、脂蛋白结果显示:与apoE2携带者(apoE2/2+E2/3+E2/4)比较,apoE3/3纯合子和apoE4携带者(apoE3/4+E4/4)血清TC、LDL-c、apoB水平均显著升高(P<0.01)。结论 apoE基因型和等位基因频率存在民族、种族差异。布依族apoE基因多态性与血脂、载脂蛋白、脂蛋白水平有关。
Objective To investigate the distribution features of apolipoprotein E （apoE） polymor- phism and analyze the influence that apoE polymorphism on lipid metabolism. Methods By using polymerase chain reaction-restriction fragment length polymorphisms（PCR-RFLPS） , blood samples of 201 subjects of Buyi ethnic group and 217 subjects of Han nationality in Qiannan, Guizhou Province were analyzed for apoE polymor- phism. The relationship of allele frequencies and serum lipids was studied. Results The frequencies of apoE genotype ε2,ε3 and ε4 of allele frequencies were 6.71%, 82.34% , and 10.95% respectively for Buyi ethnic group, and 9.22%, 84.10% and 6.68 % respectively for Han nationality （P 〉 0.05 ）. Compared with other races and nationalities, ε2, ε3 and ε4 allele frequencies were at a medium level. In both Buyi and Han nation- alities, apoE2 carriers （ apoE2/2 ＋ E2/3 ＋ E2/4）, apoE3/3 homozygote and carriers with apoE4 （ apoE3/4 ＋ E4/4） had higher serum levels of TC, LDL-C and apoB （P 〈 0.01 ）. Conclusion There is a frequency differ- ence in the apoE genotype and allele among different national/ethnic population, apoE polymorphism in Buyi ethnic minority is associated with the serum level of lipid, apolipoprotein and lipoprotein.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2012年第23期2395-2398,共4页
Journal of Third Military Medical University
基金
黔南州科学技术基金(黔南科合200707号)
黔南民族医学高等专科学校科研基金(QNYZ200602)~~
关键词
载脂蛋白E
基因多态性
布依族
血脂
载脂蛋白
apolipoprotein E
gene polymorphism
Buyi ethnic minority
serum lipid
apolipoprotein
