摘要
目的探讨鄂尔多斯地区蒙古族老年人群SLCO1B1和ApoE基因多态性分布情况,为该地区蒙古族人群基因遗传学、心脑血管疾病和阿尔茨海默病的预防、服用他汀类药物疗效和致肌病风险评估提供理论依据。方法纳入120名鄂尔多斯地区蒙古族老年健康体检居民作为研究对象,采用聚合酶链反应-荧光探针法检测其SLCO1B1和ApoE基因位点多态性。结果所有入组样本基因片段均扩增成功,共检测到7种SLCO1B1基因型,分别为*1a/*1a(5.00%)、*1a/*1b(38.33%)、*1a/*5(0.83%)、*1a/*15(10.83%)、*1b/*1b(32.50%)、*1b/*15(10.83%)、*15/*15(1.67%),未检测到*5/*5和*5/*15基因型。共检测到5种ApoE基因型,分别为ε2/ε3(5.00%)、ε2/ε4(0.83%)、ε3/ε3(80.00%)、ε3/ε4(12.50%)、ε4/ε4(1.67%)基因型,未检测到ε2/ε2基因型。结论在鄂尔多斯地区蒙古族老年人群中,SLCO1B1基因以*1a/*1b基因型和*1b等位基因最常见,ApoE基因以ε3/ε3基因型和ε3等位基因最常见。SLCO1B1基因以正常肌病风险的人群为主(75.83%),大部分人群服用他汀类降脂药物为正常肌病风险。ApoE基因以野生基因型人群为主(80.83%),大部分人群患心脑血管疾病、阿尔茨海默病为正常风险,服用他汀类药物降脂的疗效多数正常。
Objective To investigate the distribution of SLCO1B1 and Apo E gene polymorphisms in elderly Mongolian population in Ordos area,and to provide theoretical basis for genetic genetics,prevention of Cardio-cerebrovascular disease and Alzheimer’s disease,efficacy of statins and risk assessment of myopathy in elderly Mongolian population in Ordos area.Methods A total of 120 Mongolian elderly health checkup residents in the Ordos region were included as the research objects,and their SLCO1B1 and Apo E gene polymorphisms were detected by polymerase chain reactionfluorescence probe method.Results The gene fragments of all the included samples were successfully amplified,and a total of 7 SLCO1B1 genotypes were detected,namely*1a/*1a(5.00%),*1a/*1b(38.33%),*1a/*5(.83%),*1a/*15(10.83%),*1b/*1b(32.50%),*1b/*15(10.83%),*15/*15(1.67%),not detected*5/*5 And*5/*15 genotypes.A total of 5 Apo E genotypes were detected,namelyε2/ε3(5.00%),ε2/ε4(.83%),ε3/ε3(80.00%),ε3/ε4(12.50%),ε4/ε4(1.67%)Genotype,ε2/ε2 genotype was not detected.Conclusion In the Mongolian elderly population in Ordos,the SLCO1B1 gene is the most common with the*1a/*1b genotype and*1b allele,and the Apo E gene is the most common with theε3/ε3 genotype and theε3 allele.The SLCO1B1 gene is dominated by people with normal myopathy risk(75.83%),and most people taking statin lipid-lowering drugs are at normal myopathy risk.The Apo E gene is dominated by the wild genotype population(80.83%).The majority of the population suffers from cardiovascular and cerebrovascular diseases and Alzheimer’s disease as normal risks.The effect of taking statins for lowering lipids is mostly normal.
作者
陈晓晓
段宝生
CHEN Xiaoxiao;DUAN Baosheng(Baotou Medical College,Baotou,Inner Mongolia 041000;Ordos Central Hospital,Ordos,Inner Mongolia 017000)
出处
《智慧健康》
2022年第2期58-60,共3页
Smart Healthcare
