摘要
目的 :测定黄芩黄酮 A抗人肝癌细胞 BEL - 740 2体外活性及对常用抗癌药物的体外增效作用。方法 :以 MTT法测定黄芩黄酮 A的细胞毒作用及与多种抗癌药物合用后对肝癌细胞 BEL - 740 2的生长抑制作用 ,以软琼脂集落形成实验观察药物的诱导分化作用 ,以 PCR- EL ISA试剂盒测定细胞的端粒酶活性。结果 :黄芩黄酮 A能抑制肝癌细胞 BEL - 740 2的增殖 ,其IC5 0 为 180μg/ ml;黄芩黄酮 A与顺铂 ( Cis)、阿霉素 ( Dox)、氟尿嘧啶 ( 5 - FU )合用具有相加或协同作用 ,且具有诱导分化作用 ,可降低端粒酶活性的表达。结论 :黄芩黄酮 A具有体外抗肿瘤活性 ,对 Cis、Dox、5 -
AIM: To study the anticancer activity of flavone A from Skullcap on human hepatocellular carcinoma cell BEL7402 and its enhanced cytotoxicity when combined with other antitumor agents in vitro. METHODS: Cytotoxicity assay was tested by MTT method. Signs of induction differentiation were observed by soft agar clonogenic assay. Telomerase activity was determined by PCRELISA methods. RESULTS: IC 50 of flavone A from Skullcap on BEL7402 cell was 180 μg/ml. After exposure of the cells in 125 μg/ml flavone A, the inhibition ability of cisplatin, doxorubicin, and 5fluorouracil combined with flavone A were higher than that of these drugs used alone. Telomerase activity was dramatically declined during the cell incubation with flavone A. Flavone A induced differentiation of BEL7402 cell. CONCLUSION: Flavone A from Skullcap has antitumor activity in vitro and could enhance the cytotoxicity of antitumor drugs.
出处
《中国临床药学杂志》
CAS
2000年第3期166-168,共3页
Chinese Journal of Clinical Pharmacy
基金
中国-丹麦科技合作协议 Danida资助项目!(AM9:3)
关键词
黄芩黄酮A
体外抗肿瘤活性
肝肿瘤
flavone A from Skullcap
anti-tumor activity in vitro
hepatocellular carcinoma BEL-7402
drug interaction
