摘要
目的 探讨中国 2型糖尿病患者付氧酶 (PON)基因与并发冠心病 (CAD)的关系。方法利用聚合酶链反应—变性梯度凝胶电泳技术对 49例 2型糖尿病合并CAD患者、49例未合并CAD的2型糖尿病对照者和 10 1例健康对照者进行PON基因外显子筛查。结果 发现中国人群PON基因第 191位密码子存在Gln191 Arg多态性 ,等位基因以A/B表示。CAD患者PON基因的 3种基因型(AA、AB和BB)的构成比与 2型糖尿病对照和健康对照差异有显著性 ,B等位基因频率较两对照组显著增高 (0 .79比 0 .6 2和 0 .6 1,均P <0 .0 1)。结论 中国 2型糖尿病患者PON基因第 191位密码子的Gln191 Arg多态性与并发CAD有关 ,B等位基因是 2型糖尿病并发CAD的危险因素之一。
Objective To ascertain the relationship between paraoxonase (PON) gene and coronary artery disease (CAD) morbidity in Chinese type 2 diabetes mellitus (type 2 DM) patients. Methods The exons of PON gene were screened by polymerase chain reaction denaturing gradient gel electrophoresis in 49 type 2 DM patients complicated with CAD, 49 CAD uncomplicated type 2 DM as well as 101 healthy control subjects of Chinese population. Results Gln 191 Arg polymorphism of the PON gene was detected in Chinese with the A/B allele frequencies 0.39 and 0.61 respectively. The genotype distribution (AA, AB and BB) of the PON gene polymorphism was significantly different between type 2 DM patients complicated with CAD and controls (type 2 DM & healthy subjects), the former had a significantly higher B allele frequency (0.79 vs 0.62 and 0.61, P<0.01). Conclusion Gln 191 Arg polymorphism of the PON gene is associated with CAD morbidity in Chinese type 2 DM patients and B allele might be a risk factor.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2000年第1期3-5,共3页
Chinese Journal of Endocrinology and Metabolism
基金
"8 63"基金!( 10 2 10 0 2 0 3 )
上海市科委重大基础研究项目基金! ( 9741190 0 3 )
关键词
Ⅱ型
糖尿病
付氧酶
基因多态性
冠心病
Diabetes mellitus, non insulin dependent
Coronary disease
Polymerase chain reaction
Paraoxonase
Denaturing gradient gel electrophoresis
