摘要
以改良K培养液为基础,通过设定不同的N、P浓度水平,探讨N、P营养盐对克氏前沟藻Amphidiniumklebsii(三亚株)生长及产毒特性的影响。研究结果表明,N、P均为影响克氏前沟藻生长和产毒的重要因素。低浓度的N、P(N浓度≤4.665×10 4mol.L 1,P浓度≤2.000×10 5mol.L 1)不利于克氏前沟藻生物量的积累,但能促进其生长及毒素的产生;中浓度的N、P(4.665×10 4mol.L 1<N浓度<37.320×10 4mol.L 1,2.000×10 5mol.L 1<P浓度<16.000×10 5mol.L 1)有利于克氏前沟藻生物量的积累,却不利于毒素的产生;N、P浓度过高(N浓度≥37.320×10 4mol.L 1,P浓度≥16.000×10 5mol.L 1)则会抑制克氏前沟藻的生长,但能促进毒素的产生,并有相对较高的细胞终密度。克氏前沟藻生长的最佳N、P浓度范围分别为4.665×10 4—37.320×10 4mol.L 1和2.000×10 5—16.000×10 5mol.L 1。当N浓度为9.330×10 4mol.L 1,P浓度为4.000×10 5mol.L 1时,克氏前沟藻达到最高密度1.237×105mL 1,单位体积活血活性达到最高值347.48HU.L 1。
In order to evaluate the growth and toxin production of Amphidinium klebsii（Sanya strain） under different nitrate and phosphate concentrations,a series of nitrate and phosphate solutions were prepared based on modified K medium.Nitrate and phosphate had significant effects on the growth and toxin productivity of A.klebsii.Low-nitrate and low-phosphate concentrations（N concentration≤4.665×10 4mol.L 1,P concentration≤2.000×10 5mol.L 1） in culture media were not favorable to the biomass accumulation of A.klebsii but favorable to its growth and toxin production.On the contrary,middle-nitrate and middle-phosphate concentrations（4.665×10 4mol.L 1N concentration37.320×10 4mol.L 1,2.000× 10 5mol.L 1P concentration16.000×10 5mol.L 1） were favorable to the biomass accumulation of A.klebsii but not favorable to its toxin production.Extra-high-level nitrate and phosphate concentrations（N concentration ≥ 37.320 × 10 4mol.L 1,P concentration≥16.000×10 5mol.L-1） seemed to inhibit the growth of A.klebsii,but resulted in higher cell densities and contributed to its toxin production.Under the experimental condition,optimum growth N and P concentrations were 4.665×10 4mol.L 1N concentration37.320×10 4mol.L 1,2.000×10 5mol.L 1P concentration16.000×10^ 5mol.L^-1.The highest biomass value estimated in culture was 1.237×105mL 1 at the levels of nitrate 9.330×10^–4mol.L^–1 and phosphate 4.000×10^–5mol.L^–1,and the maximum hemolytic activity reached 347.48HU.L 1 under the same condition.
出处
《热带海洋学报》
CAS
CSCD
北大核心
2012年第4期117-123,共7页
Journal of Tropical Oceanography
基金
中国科学院重要方向项目(KSCX2-YW-R-093)
海洋公益性行业科研专项(200705026)
关键词
克氏前沟藻
溶血毒素
氮
磷
Amphidinium klebsii
haemolytic toxin
nitrate
phosphate
