摘要
目的:建立一种稳定、准确测定大鼠血浆中β-榄香烯的固相萃取-高效液相色谱检测方法,为进一步系统开展该化合物体内药代动力学研究奠定方法学基础。方法:β-榄香烯血浆样品经固相萃取柱萃取后,采用高效液相色谱法检测,色谱柱为WatersNavpack C18柱(150×3.9mmi.d.,5μm),柱前接Waters C18预柱(8×4mm,i.d.,5μm),流动相为乙腈:水(90:10,v/v),流速1.0 mL.min-1,柱温30℃,检测波长210nm,内标为蓬莪术环二烯。结果:大鼠血浆中β-榄香烯含量的线性范围为0.147～5.88μg.mL-1,R=0.998,。方法日内精密度与日间精密度均≤15%,萃取回收率≥71%。结论:本研究建立的β-榄香烯血药浓度测定方法有较高的准确度和灵敏度,适用于该化合物的体内药代动力学研究。
The purpose of this study was to develop a simple and sensitive high performance liquid chromatography（HPLC） method for the quantification of β-elemene,a novel antineoplastic drug,in rat plasma.β-elemene and furanodiene（internal standard,IS） were successfully separated on a Waters Navpack C18 column（150×3.9mm i.d.,5 μm） equipped with a C18 guard column（8×4mm,i.d.,5 μm） and a mobile phase of acetonitrile-water（90：10,v/v） at a flow rate of 1.0mL·min-1.High extraction recovery（71%） for both β-elemene and IS was obtained by a rapid and efficient solid-phase extraction.The calibration curve was linear in range from 0.147 to 5.88 μg·mL-1 with excellent correlation coefficients（R=0.998）.Intra-day and inter-day precision were less than 15% over the entire linear range.The validated method was sensitive and accurate.It was suitable for the application of the pharmacokinetics ofβ-elemene in rat.
出处
《科技通报》
北大核心
2012年第11期87-90,共4页
Bulletin of Science and Technology
基金
浙江省重点科技创新团队项目(项目编号:2012R10044-07)
浙江省医药卫生科技项目(项目编号:2011KYA119)
