摘要
目的研究建立奥利司他超高效液相色谱快速检测方法。方法以ACQUITY UPLC BEH C18柱(Φ100mm×2.1mm,1.7μm)为固定相,以81%乙腈水溶液为流动相,流速0.4mL.min-1,进样0.8μL,在195nm检测,采集信号并分析。结果在此色谱条件下,可有效地对奥利司他样品进行快速检测。结论该超高效液相色谱法可快速、有效地完成对奥利司他的分析检测。
Objective To develop an UPLC method for the determination of orlistat.Method Orlistat was separated with ACQUITY UPLC BEH C18 column（Φ100 mm×2.1 mm,1.7 μm）,using 81% acetonitrile solution as mobile phase,and detected at 195 nm.The flow rate was 0.4 mL·min-1,and injection volume was 0.8 μL.Result In the given chromatography conditions,orlistat can be determined efficiently.Conclusion This method can be used for the determination of orlistat samples effectively and efficiently.
出处
《西北药学杂志》
CAS
2012年第6期533-535,共3页
Northwest Pharmaceutical Journal
基金
广东省产学研项目(省部特派员工作站依托项目)
项目编号:2010B090500047
