摘要
目的对高效液相色谱仪器和标准物质进行期间核查。方法使用《化妆品安全技术规范》(2015)中的测定方法,对高效液相色谱仪及4℃冰箱保存6个月后的氯咪巴唑标准物质采用留存的样品(去屑洗发香波)再测的方式进行期间核查,并用En值进行评价。结果高效液相色谱仪核查的参数分别是定性测量重复性、定量测量重复性及测定的准确性,其中定性测定重复性用保留时间的RSD表示为0.59%,小于要求的1%,结论为符合要求;定量测量重复性用峰面积的RSD表示为0.28%,小于要求的3%,结论为符合要求;测定的准确性用En值表示为0.18,小于要求的1,结论为符合要求。标准物质核查参数是量值核查,用En值表示为0.18,小于要求的1,结论为符合要求。结论使用留样再测和En值评价的方式可以同时实现高效液相色谱仪与标准物质的期间核查。这样可以节约检测成本同时还简化了操作,适用于实验室的仪器和标准物质的常规期间核查。
Objective To perform an intermediate check of high performance liquid chromatograph（ HPLC） and reference materials. Methods By retesting sample,the intermediate check of high performance liquid chromatograph and climbazole reference at 4 ℃ in refrigerator for 6 months was carried out according to Technical Specification for Cosmetic Safety（ 2015） and the results were evaluated by the En value. Results The parameters of the high performance liquid chromatograph check were qualitative measurement repeatability,quantitative measurement repeatability and measurement accuracy,respectively. The qualitative measurement repeatability was represented by the relative standard deviation（ RSD） of the retention time which was0. 59%,less than 1%,so it was eligible. The quantitative measurement repeatability was represented by the RSD of the peak area which was less than 3%,so it was eligible too. The accuracy of the determination was represented by the En value,which was 0. 18,less than 1,so it was eligible as well. Conclusion Using retesting sample and En value evaluation,the intermediate check of high performance liquid chromatograph and reference materials could be carried out simultaneously. It could save the cost of determination and simplify operation procedures and meet the needs for routine intermediate check of instruments and reference materials in laboratory.
出处
《中国卫生检验杂志》
CAS
2017年第20期3016-3018,共3页
Chinese Journal of Health Laboratory Technology
关键词
高效液相色谱
标准物质
期间核查
High performance liquid chromatograph
Reference materials
Intermediate check
