摘要
目的探讨免疫因子相关基因白细胞介素-2受体仅链（IL-2RA）rs2104286和白细胞分化抗原40（CD40）rs6074022多态性与急性一氧化碳中毒后迟发性脑病（DEACMP）之间的关联。方法选择豫北地区汉族DEACMP患者109例作为研究组，急性一氧化碳中毒（ACUte）未发生迟发脑病患者115例作为对照组，采用PCR．限制性片段长度多态性和荧光定量PCR技术，检测rs2104286和rs6074022的基因型，两组间的比较采用x^2检验。结果（1）DEACMP组rs2104286基因型（rr／T、T／C、C／C）和等位基因（T、C）分别为92例，17例，0例和201例，17例，ACMP组分别为93例，22例，0例和208例，22例，两组比较均差异无统计学意义（均P〉0．05）。DEACMP组rs6074022基因型（C／C、C／T、T／T）和等位基因（C、T）分别为17例，50例，42例和84例，134例，ACMP组分别为15例，52例，48例和82例，148例，两组比较均差异无统计学意义（均P〉0．05）。（2）按性别分层后，两组女性患者rs2104286及rs6074022基因型和等位基因比较均差异无统计学意义（均P〉0．05），两组男性患者rs2104286及rs6074022基因型和等位基因比较均差异无统计学意义（均P〉0．05）。（3）rs2104286及rs6074022基因不同基因型对DEACMP的发生无相互作用（均P〉0．05）。结论尚未发现rs2104286和rs6074022基因多态性与DEACMP关联的证据，暂不支持IL-2RA和CD40基因作为DEACMP的遗传易感基因。
Objective To study the association between interleukin-2 receptor 2 chain （IL-2RA） rs2104286,one of immume-factor-related genes,and leukocyte differentiation antigen 40 （CD40） rs6074022 gene polymorphism and delayed encephalopathy after acute carbon monoxide poisoning （DEACMP）. Methods 109 DEACMP patients from Han population in Northern Henan Province were selected as the case group, and 115 patients of acute carbon monoxide poisoning without delayed encephalopathy （ACMP） as the control group; rs2104286 and rs6074022 genotypes were determined with PCR-restriction fragment length polymorphism （PCR- RFLP） and fluorescence quantitative PCR. The comparison was represented by X2 test. Results （1）In DEACMP group, the rs2104286 genotypes （ T/T, T/C, C/C ） and alleles （ T and C ） were 92,17,0 and 201,17, respectively. In contrast to 93,22,0 and 208,22 in ACMP group, no significant difference was noticed （ all P 〉 0.05 ） , and the rs6074022 genotypes （C/C, C/T,T/T） and alleles （C and T） were 17,50,42 and 84,134, respectively. In con- trast to 15,52,48 and 82,148 in ACMP group,no significant difference was noticed （all P〉0.05）. （2） With re- gards to gender stratification, the rs2104286 and rs6074022 geuotypes and alleles of female patients in both groups were not significantly different （all P〉 0.05 ） ; the rs2104286 and rs6074022 genotypes and alleles of male patients in both groups were not significantly different （ all P 〉 0.05 ）. （3） No interactions between different genotypes of rs2104286 and rs6074022 and DEACMP occurrence were observed （all P〉0.05） ,which indicated that the above interactions might not impact on the genetic susceptibility of DEACMP. Conclusion No evidence has yet shown the association between rs2104286 and rs6074022 gene polymorphism with DEACMP; IL-2RA and CD40 genes as genetic susceptibility genes of DEACMP are not temporarily supported.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2012年第11期985-987,共3页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家自然科学基金资助项目（81141071）
河南省科技厅科技攻关重点资助项目（102102310152）
新乡医学院省重点学科校内开放课题资助项目（ZD200996）
新乡医学院研究生科研创新支持计划资助项目（YJSCX201108Z）
关键词
一氧化碳中毒
迟发脑病
白细胞介素-2受体α链
白细胞分化抗原40
Carbon monoxide poisoning
Delayed encephalopathy
Interleukin-2 receptor a chain
Leukocyte differentiation antigen 40
