摘要
应用SRAP和SSR分子标记对8份辣椒种质进行了遗传多样性分析,结果表明,15对SRAP引物组合共扩增出321条带,平均每对引物扩增出21.40条,多态性位点比率为72.90%;18对SSR引物共扩增出109条带,平均每对引物扩增出6.06条,多态性位点比率为98.17%。与SRAP比较,SSR检测到的Shannon多样性指数(I)、观测等位基因数(Na)和有效等位基因数(Ne)等遗传多样性参数都较大,说明SSR有更高的多态性检测效率。基于SRAP的聚类与基于SSR的聚类之间存在极显著正相关,且都能将中国灌木辣椒种质与美洲灌木辣椒种质及一年生辣椒种质有效区分。
Eight accessions in pepper were analyzed with SRAP and SSR markers. Fifteen SRAP primers and eighteen SSR primers generated 321 and 109 fragments, respectively. The number of fragments per primer, the percentage of polymorphic loci was in turn 21.40,72.90% for SRAP markers,and 6.06,98.17%for SSR markers. Shannon＇s information index （I） ,observed number of alleles （Na） and effective number of alleles （Ne） based on SSR markers were bigger than those based on SRAP. The results showed that SSR markers could detect more genetic diversity in the pepper accessions. The correlation analysis among genet- ic similarities based on SRAP and SSR data were significantly correlated. Both SRAP and SSR dendrograms could separate Capsicum frutescens accessions in China from the same species accession in American and C. annuum accession.
出处
《西北植物学报》
CAS
CSCD
北大核心
2012年第11期2201-2205,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(31060203)
国家自然科学基金(30860173)
