摘要
用RAPD技术对我国的34个辣(甜)椒品种进行了分析,22个随机引物共扩增出119条带,其中67条具多态性。采用Nei、Jaccard和欧氏距离3种方法计算各品种间的遗传距离矩阵,分别根据3个遗传距离矩阵进行UPGMA聚类分析,得出3个树形聚类图。聚类结果表明,IBPGR将辣椒种划分为4个变种的建议更为合理,Nei方法与Jaccard方法的结果均认为圆锥椒与灯笼椒的亲缘关系较近,而欧氏距离法的结果则认为所有辛辣类型的亲缘关系较近。研究还发现,栽培及育种活动在变种的发展过程中起到了重要作用。
RAPD analysis was employed to analyze 34 chili (pimiento) varieties and its 22 random primers amplified 119 bands of which six seven were polymorphic.Nei's method,Jaccard's method,and Euclidean distance method were adopted to calculate the matrixes of the genetic distances of various varieties and then three tree diagrams were constructed in accordance with the matrixes in UPGMA cluster analysis.The cluster analysis revealed that IBPGR's chili classification that Capsicum annuum could be divided into four varieties was relatively reasonable compared with those by Nei's method and Jaccard's method that var.conoides had close genetic relation with var.grossum,and that by Euclidean distance method that all chili types had close genetic relations.In addition,it was found that farming practices and breeding had played an important role in the developments of these varieties.
出处
《西北植物学报》
CAS
CSCD
北大核心
2005年第5期870-875,共6页
Acta Botanica Boreali-Occidentalia Sinica
