摘要
采用EDTA-Na2法提取硫琼脂,采用硫酸钡比浊法及苯酚-硫酸法分别测定硫琼脂的硫酸根含量和总糖含量。在单因素试验的基础上,利用响应曲面法优化提取工艺,建立了EDTA-Na2加入量(A)、洗脱时间(B)和洗脱温度(C)与硫琼脂的主要理化指标(硫酸根+总糖)含量(Y)之间的数学模型,确定了硫琼脂提取的最佳工艺条件为:EDTA-Na2加入量为5.90g.L-1、洗脱时间2.2h、洗脱温度为53℃。按此最佳工艺提取的硫琼脂的主要理化指标(硫酸根+总糖)含量为(84.00±0.45)%。
Agaropectin was extracted by EDTA-Na2, the content of sulfate group and total sugar were determined by barium sulfate turbidimetry and hydrochloric acid and phenol-sulfuric acid method, respectively. Based on single factor experiments, the extraction technique for agaropectin was optimized using response surface methodology, the mathematical regression model was established for predicting the value of sulfate group content plus total sugar for agaropectin （Y） at different levels of adding EDTA-Naz （A）, treating time （B） and temperature （C）. The optimal extraction parameters for agaropectin were determined as： adding EDTA-Na2 5.90 g .L-1 , treating time 2.2 h at temperature of 53℃, respectively. With the optimal parameters, the content of sulfate group plus total sugar for agaropectin extracted was 84.00±0.04%, which was in good agreement with the predicted value （Y）.
出处
《福建农业学报》
CAS
2012年第9期1004-1010,共7页
Fujian Journal of Agricultural Sciences
基金
国家海洋公益性行业科研专项(201205022-4)
关键词
硫琼脂
提取工艺
响应面法
agaropectin
extraction technology
response surface methodology
