摘要
目的观察胺碘酮对兔心肌缺血再灌注炎症因子水平的影响。方法 30只新西兰大白兔随机分为模型对照组、胺碘酮组和假手术组,每组10只。模型对照组和胺碘酮组建立缺血再灌注无复流模型,分别于再灌注后120min取血,采用酶联免疫吸附法(ELISA)测定兔血清超敏C反应蛋白(hs-CRP)、血栓素A2(TXA2)、5-羟色胺(5-HT)水平,并进行组间比较。结果在缺血范围差异无统计学意义的条件下,胺碘酮组无复流面积明显小于模型对照组[(50.44±5.36)%vs.(78.91±3.35),P<0.01]。胺碘酮组血清hsCRP、TXA2、5-HT水平(2.80±0.18)μg/L、(3.27±0.54)mg/L、(2.63±0.73)ng/L明显低于模型对照组(3.05±0.29)μg/L、(4.57±0.78)mg/L、(3.84±0.93)ng/L,差异均有统计学意义(P<0.05)。结论胺碘酮可抑制兔急性缺血再灌注后炎症反应,减轻心肌无复流损伤程度。
Objective To observe the influence of amiodarone on inflammatory factor level in rabbits with acute myocardial ischemia-reperfusion. Methods New Zealand rabbits （ n =30 ） were randomly divided into model control group, amiodarone group and sham-operation group （ each n =10 ） .The model of ischemiareperfusion non-reflowing was established in model control group and amiodarone group. The blood samples were collected after reperfusion for 120 minutes. The levels of serum high sensitivity C-reactive protein （ hsCRP ） , thromboxane A2 （ TXA2 ） and 5-hydroxytryptamine （ 5-HT ） were detected by using enzyme-linked immunosorbent assay （ ELISA ） and compared among all groups. Results Under the condition of the difference in ischemic areas without statistical significance, the non-reflowing area was significantly smaller in amiodarone group than that in model control group [ （ 50.44 ± 5.36 ） % vs. （ 78.91 ± 3.35 ） , P 0.01]. The levels of serum hs-CRP, TXA2 and 5-HT were significantly lower in amiodarone group [ （ 2.80 ± 0.18 ）μ g/L, （ 3.27 ± 0.54 ） mg/L, （ 2.63 ± 0.73 ） ng/L] than those in model control group [ （ 3.05 ± 0.29 ）μ g/L, （ 4.57 ± 0.78 ） mg/L, （ 3.84 ± 0.93 ） ng/L] with significant statistical difference （ P 0.05 ） . Conclusion Amiodarone can inhibit the inflammatory reaction after ischemia-reperfusion and reduce myocardial non-reflowing lesion in rabbits with acute ischemia-reperfusion.
出处
《中国循证心血管医学杂志》
2012年第5期473-475,共3页
Chinese Journal of Evidence-Based Cardiovascular Medicine
基金
河北省科技计划项目(09276101D-8)
关键词
炎症因子
胺碘酮
缺血
再灌注
无复流
兔
Inflammatory factors
Amiodarone
Ischemia-reperfusion
Non-reflowing
rabbits
