摘要
采用＾３Ｈ－ＴｄＲ掺入法和酸性醋酸萘酯酶活性检测法分别研究了草鱼外周血以及免疫器官中的淋巴细胞对ＰＨＡ和ＳＰＡ的免疫应答以及化学标志特征，发现草鱼外周血中存在着类似于哺乳动物的Ｔ、Ｂ两类不同的淋巴细胞亚群；同时也发现胸腺中只有Ｔ淋巴细胞、而无Ｂ淋巴细胞，提示了胸腺中淋巴细胞可能是外周血中Ｔ细胞的来源，而Ｂ淋巴细胞可能来自脾脏或前肾。
The studies on the capacities of immune responses of lymphocytes in peripheral blood and immune organs of Grass Carp (Cteapharyngoden idellus Cuvier et Valencinnes) to PHA and SPA with incorporation of ~3H-TdR and the active determine of Acid a-Naphthyl Acetate Esterase (ANAE) indicated that there were T and B lymphocytes in peripheral blood of grass carp, but only T lymphocyte and no B lymphocytes in the thymus of grass carp. It was justified that the T lymphocyte in peripheral blood originated from the thymus, and B Iymphocytes could originated from the spleen and anterior kidney of grass carp.
基金
浙江省自然科学基金!(395185)
关键词
草鱼
淋巴细胞
异质性
免疫应答
酸性醋酸萘酯酶
Grass Carp (Ctenpharyngoden idellus Cuvier et Valencinnes)
T and B lymphocytes
thymus
spleen
anterior kidney
