摘要
目的:检测结直肠癌患者外周血循环中黑素瘤抗原编码基因(melanoma antigen encoding gene,MAGE)-A3、MAGE-A6和MAGE-A10的表达情况,并分析其临床意义。方法:采用实时荧光定量PCR(real-time fluorescent quantitative PCR,RFQ-PCR)检测35例结直肠癌患者和15例正常对照者外周血中MAGE-A3、MAGE-A6和MAGE-A10mRNA的表达水平,并分析其与结直肠癌患者临床病理特征(性别、年龄、肿瘤部位、组织学分级、肿瘤浸润深度、淋巴结转移和Dukes分期)之间的关系。结果:所有外周血样本中均未检测出MAGE-A3mRNA的表达。结直肠癌组MAGE-A6mRNA表达阳性率显著高于正常对照组(P=0.0000),男性患者的表达水平高于女性患者(P=0.0459),淋巴结转移患者的表达水平也高于无转移患者(P=0.0000);结直肠癌组MAGE-A10mRNA表达阳性率也显著高于正常对照组(P=0.0224);淋巴结转移患者的表达水平也高于无转移患者(P=0.0000)。结论:结直肠癌患者外周血循环中MAGE-A6和MAGE-A10mRNA的表达在结直肠癌转移检测中具有重要意义,可能成为结直肠癌微转移诊断、治疗选择和预后判断中的重要临床靶标。
Objective： To analyze the mRNA expression levels of MAGE （melanoma antigen encoding gene）-A3, MAGE-A6 and MAGE-A10 in peripheral blood of patients with CRC （colorectal carcinoma）, and to study their clinical significance. Methods： Peripheral blood samples were collected from 35 CRC patients and 15 healthy donors. RFQ-PCR （real-time fluorescent quantitative PCR） was performed to detect the mRNA expression levels of MAGE-A3, MAGE-A6 and MAGE-A10 for all samples. The correlation of clinicopathological characteristics （gender, age, primary tumor lesion, histologic grading, depth of invasion, lymph node metastasis, Dukes’ staging） with the expressions of MAGE-A6 and MAGE-A10 mRNAs in peripheral blood of patients with CRC （colorectal cancer） was evaluated. Results： MAGE-A3 mRNA expression was not detected in all peripheral blood samples. The positive rate of MAGE-A6 mRNA in CRC group was significantly higher than that in healthy control group （P = 0.000 0）, and its expression level in CRC male patients was significantly higher than that in female patients （P = 0.045 9）. Furthermore, MAGE-A6 mRNA was more significantly expressed in CRC patients with lymph node metastasis compared with the patients without lymph node metastasis （P = 0.000 0）. The positive rate of MAGE-A10 mRNA in CRC group was also significantly higher than that in healthy control group （P = 0.022 4）, and the expression level in CRC patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis （P = 0.000 0）. Conclusion： The mRNA expression level of MAGE-A6 and MAGE-A10 in peripheral blood of CRC patients shows an important significance for CRC metastasis detection, and it may become a potential clinical marker for the diagnosis of micro-metastasis in lymph nodes, treatment selection and prognostic judgement of CRC.
出处
《肿瘤》
CAS
CSCD
北大核心
2012年第11期913-918,共6页
Tumor
基金
上海浦东新区重大科技专项资金项目(编号:PKJ2008-38)
上海浦东新区学科带头人培养计划(编号:PEWd2010-05)
关键词
结直肠肿瘤
循环肿瘤细胞
黑素瘤抗原
实时荧光定量PCR
Colorectal neoplasms
Circulating tumor cells
Melanoma antigen
Real-time fluorescentquantitative PCR
