摘要
目的探讨抗栓中药对过氧化氢（H2O2）诱导人脐静脉内皮细胞（HUVEC）氧化损伤的保护作用。方法实验分为正常对照组、氧化损伤模型组及血栓心脉宁片给药组．以H2O2致HUVEC的氧化损伤模型为研究对象，加入抗栓中药血栓心脉宁片进行预先干预。采用甲基偶氮四唑蓝（MTT）法检测细胞活力，流式细胞术（FCM）检测细胞的生长周期，酶联免疫吸附试验（ELISA）检测细胞一氧化氮（NO）、一氧化氮合酶fNOS）的含量及超氧化物歧化酶（SOD）的活性。结果与正常对照组相比，氧化损伤模型组MTT检测结果显示HUVEC活力明显下降，FCM检测结果显示细胞生长周期阻滞在G0-G1期，且ELISA结果显示细胞上清液中NO含量及NOS含量及SOD活性显著降低，NO含量由31．95μmol／L降低到24．82μmol／L，NOS含量由3．13μmol／L降低到2．58μmol／L，SOD活性由128．2U／mL降低至109．17U／mL，差异均具有统计学意义（P〈0．05），表明HUVEC氧化损伤明显。与氧化损伤模型组相比，血栓心脉宁片给药组细胞生长在GO—G1期没有出现阻滞作用，细胞上清液中NO、NOS含量及SOD活性均明显增加，差异有统计学意义（P〈0．05）。结论FCM和ELISA研究结果表明抗栓中药血栓心脉宁片具有明显的抗HUVEC氧化损伤作用。
Objective To investigate the protective effect of antithrombotic traditional Chinese medicine on human umbilical vein endothelial cell （HUVEC） injury induced by hydrogen peroxide （H2O2）. Methods Normal control group, oxidative damage model group and Xueshuan Xinmaining tablet treatment group were chosen in our study. The oxidative injured HUVEC models were established by using H2O2. Intervenes before treatment were made by adding antithrombotie traditional Chinese medicine Xueshuan Xinmaining tablets. Cell viability and cell growth cycle changes were observed by 3- （4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） staining and flow cytometry （FCM）, respectively. ELISA was employed to detect the contents of Nitric oxide （NO） and nitric oxide synthase （NOS）, as well as superoxide dismutase （SOD） level. Results MTT results showed that the cell activity of HUVECs in the oxidative damage model group was significantly decreased as compared with that in the control group; cell growth of the oxidative damage cells was blocked in the G0-G1 period detected by FCM; and ELISA showed that the levels of NO and NOS were decreased significantly and the activity of SOD was decreased in the oxidative damage model group as compared with those in the normal control group （P〈0.05）; the level of NO was decreased from 31.95 μmol/L to 24.82 μmol/L and the level of NOS was decreased from 3.13 μmol/L to 2.58 μmol/L; the activity of SOD was decreased from 128.2 U/mL to 109.17 U/mL; these results showed that HUVEC oxidative damage was obvious. Xueshuan Xinmaining tablet treatment could resist the cell injure induced by H2O2 without obvious cell block at G0-G1 periods, and NO and NOS levels were increased significantly and the activity of SOD was increased as compared with those in the oxidative damage model group （P〈0.05）. Conclusion FCM and ELISA results show that antithrombotic traditional Chinese medicine has obvious protective effect on HUVEC oxidative injury.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2012年第11期1138-1142,共5页
Chinese Journal of Neuromedicine
基金
吉林省科技发展计划项目（201205014）
关键词
抗栓中药
人脐静脉内皮细胞
氧化损伤
过氧化氢
Antithrombotic traditional Chinese medicine
Human vein umbilical endothelial cell
Oxidative injury
Hydrogen peroxide
