摘要
对桦褐孔菌深层发酵培养基进行了筛选,以菌丝体及甾类化合物产量为目标对发酵条件进行了优化,确定最佳发酵条件为:30g/L葡萄糖,2.5g/L黄豆粉,2.5g/L蛋白胨,3g/LKH2PO4,0.8g/LMgSO4,0.8g/LCaSO4,初始pH4.0,接种量15%,装液量100mL/500mL,转速150r/min,28℃恒温培养。此条件下培养11d,菌丝体干重达12.52g/L,甾体类化合物的产量达112.44mg/L。
To produce mycelial biomass and steroids, the optimization of submerged fermentation condition for Inonotus obliquus was carried out. The optimum fermentation medium was as follows： 30g/L glucose, 2.5g/L Soybean powder, 2.5g/L peptone, 3g/L KH2PO4, 0.8g/L MgSO4, 0.8g/L CaSO4. The initial pH was 4.0, and the inoculation volume was 15%. The fungus was cultured at 28℃ in 500mL shake flasks containing 100mL of medium at 150r/rain. Under this condition, mycelial biomass of 12.52g/L was obtained while production of steroids about 112.44mg/L.
出处
《菌物学报》
CAS
CSCD
北大核心
2012年第6期909-916,共8页
Mycosystema
基金
国家"863"重点项目(No.2011AA02A211)
江苏省自然科学基金(No.BK2010142)
江苏省产学研联合创新资金项目(No.BY2012052)
中央高校基础研究项目(Nos.JUSRP211A42
JUSRP211A43)
关键词
桦褐孔菌
最佳发酵培养基
初始pH
接种量
Inonotus obliquus, optimum fermentation medium, initial pH, inoculation volume
