摘要
目的:建立以高效液相色谱法测定肝毒净丸中白藜芦醇苷、岩白菜素含量的方法。方法:C18柱(4.6 mm×150 mm,5μm),流动相分别为乙腈-水(20∶80)、甲醇-水(20∶80),检测波长分别为303,275 nm,流速1.0 mL·min-1。结果:白藜芦醇苷在0.121 2～0.323 2μg(r=0.999 9)线性关系良好,平均加样回收率97.99%,RSD 0.69%;岩白菜素在0.148 5～0.396μg(r=0.999 9)线性关系良好,平均加样回收率98.65%,RSD 0.92%。结论:方法操作简便、准确、重复性好,为肝毒净丸的质量控制提供了依据。
Objective： to build a high performance liquid chromatography on determination of the polydatin and bergenin in Gandujing Pill. Method： Cl8 column （4.6mm×150mm,5μm） , mobile phase of acetonitrile-water （20： 80）, methanol-water （20： 80）, wavelength detection at 303, 275 nm, flow rate 1.0 mL. min-I Result： Polydatin showed a good linear relationship in the range of 0. 121 2-0. 323 2 μg （r = 0. 999 9） ; the average recovery was 97.99% with RSD of 0.69%. Bergenin showed a good linear relationship in the range of 0. 148 5-0. 396 μg （ r= 0. 999 9） ; the average recovery was 98.65% with RSD of 0. 92%. Conclusion： The method with good repeatability is simple and accurate, which provides a basis for quality control of the Gandujing Pill.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第22期149-151,共3页
Chinese Journal of Experimental Traditional Medical Formulae
