摘要
目的研究大气细颗粒物对大鼠肺组织亚急性作用及其可能的作用机制。方法健康成年雄性Wistar大鼠35只随机分为空白对照组、生理盐水对照组、低剂量染尘组(1.5mg·kg-1·bw-1)、中剂量染尘组(7.5mg·kg-1·bw-1)、高剂量染尘组(37.5mg·kg-1·bw-1)。空白对照组不采取任何处理措施,生理盐水对照组用生理盐水进行气管滴注,将采集细颗粒物制成所需浓度的生理盐水混悬液对各剂量染尘组进行气管滴注。测定血清及灌洗液(BALF)中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、丙二醛(MDA)含量。结果空白对照组与生理盐水对照组各测定指标差异无统计学意义(P>0.05)。血清和灌洗液(BALF)中,各染尘组指标ACP、AKP、LDH、MDA与生理盐水对照组相比差异均有统计学意义(P<0.05),且存在剂量依赖关系。结论环境细颗粒物亚急性染尘可对大鼠肺组织造成一定程度的损伤作用。
Objective To explore the subacute oxidation injury effect and the possible mechanisms of ambient fine particulates on rats′ lung.Methods 35 healthy,adult and male Wistar rats were randomly divided into five groups including blank control group,saline control group,and three levels PM 2.5 groups.The concentrations of three level groups were 1.5 mg/kg,7.5 mg/kg and 37.5 mg/kg respectively.The blank group was not taken any measures.The collected particulates were suspended in saline.All experimental groups were inoculated with suspensions of PM 2.5 via trachea and saline control group were inoculated saline.The blood serum and bronchoalveolar lavage fluid（BALF） of all mice were later examined,including the level of ACP,AKP,LDH,MDA.Results All indexes were no significantly difference between blank control group and saline control group.In serum and BALF,three level group′s activities of LDH,ACP and AKP and content of MDA were both higher than control groups.The dose response relationship was observed in all indexes.Conclusion The ambient particulates could have definite injury effect on rats′ lung.
出处
《新疆医科大学学报》
CAS
2012年第11期1500-1503,共4页
Journal of Xinjiang Medical University
关键词
细颗粒物
亚急性
大鼠
实验研究
fine particulate
subacute
rats
experiment study
