摘要
目的:建立姜黄素白蛋白纳米混悬剂包封率的测定方法。方法:通过离心法分离出游离的药物,利用紫外分光光度法,在422nm波长处测定姜黄素含量,计算姜黄素白蛋白纳米混悬剂的包封率。结果:姜黄素检测浓度在0.5～6.0mg.L-1范围内与吸光度呈良好线性关系(r=0.9999);平均加样回收率为98.50%,RSD=2.74%(n=9);平均包封率为42.32%。结论:该方法操作简便、精密度好、结果可靠,可用于姜黄素白蛋白纳米混悬剂包封率的测定。
OBJECTIVE：To establish the method for the determination of encapsulation efficiency of Curcumin albumin nanosuspensions.METHODS：Centrifuging was applied to isolate free drug and the content of curcumin was determined at 422 nm by UV spectrophotometry.The encapsulation efficiency of Curcumin albumin nanosuspensions was calculated.RESULTS：Curcumin showed a good linear relationship within a range of 0.5～6.0 mg·L-1（r=0.999 9） with an average recovery of 98.50%（RSD=2.74%,n=9）.The encapsulation efficiency was 42.32%.CONCLUSION：The method is easy to operate,highly accurate,reliable in results and can be used for the determination of encapsulation efficiency of Curcumin albumin nanosuspensions.
出处
《中国药房》
CAS
CSCD
2012年第43期4068-4070,共3页
China Pharmacy
