摘要
目的建立右旋布洛芬-β-环糊精包合物的含量环糊精包合物中右旋布洛芬的含量测定方法。方法用75％的甲醇溶解样品，超声处理30min，稀释制成每1ml中约含右旋布洛芬36．5ug的溶液。采用HPLC法，色谱柱：日本岛津VP-ODS柱（150mm×4．6mm，5um）；流动相：甲醇-0．2％磷酸溶液（75：25），检测波长：220nm。结果右旋布洛芬在10．01～50．05g·L^-1范围内呈良好的线性关系（r=0．9999），平均回收率为98．71％，RSD=1．38％（n=9）。结论该方法简便、准确、重现性好。适用右旋布洛芬包合物中主药的含量测定。
Aim To establish an HPLC method for the determination of dexibuprofen in dexibuprofen-β-cyclodextrin inclusion complex. Methods The sample was dissolved with 75% methyl alcohol and prepared by uhrasonieation. Its concentration was diluted into about 36.5g·L^-1. Then it was diluted to 36.5g·L^-1 dexibuprofen. A Shim-pack VP-ODS column was used. The mobile phase was methyl alcohol-0.2% phosphoric acid solution（75：25 ）. The detection wavelength was set at 220 nm. Results The linear range of dexibuprofen was 10.01 -50.05g·L^-1（ r = 0.9 999 ）. The average recovery rate was 98.71% （RSD = 1.38% ）. Conclusion The method is simple, accurate and repeatable which can be used for the determination of dexibuprofen in dexibuprofen-β-eyclodextrin inclusion complex.
出处
《安徽医药》
CAS
2009年第2期157-158,共2页
Anhui Medical and Pharmaceutical Journal
