摘要
分析了植物组织培养过程中的污染原因,结合外植体消毒技术的研究现状,提出如何控制由外植体引发污染的方法。选择合适的外植体和消毒剂,采用联合消毒方法,对外植体初代培养中内源污染的控制效果比较明显。另外,对于难消毒的外植体,配合使用抗生素能有效地控制污染。
In this paper, the reason of the pollution in plant tissue culture was analyzed. Combined with the research on the disinfection technology of explants, the method of controlling the pollution caused by the explants was proposed. Selecting the appropriate explants and disinfectants, and using the combined disinfection method, the pollution control of the explants on the initially cultured was effective. In addition, to the explants which were difficultly disinfected, cooperatively using the antibiotics could effectively control the pollution.
出处
《热带农业科学》
2012年第9期53-56,共4页
Chinese Journal of Tropical Agriculture
基金
中央级公益性科研院所基本科研业务费中国热带农业科学院橡胶研究所基本科研业务费专项(No.1630022011005)
现代农业产业技术体系建设专项(No.NYCYTX-34)
关键词
植物组织培养
污染
外植体
消毒
控制
plant tissue culture
pollution
explant
disinfection
control
