摘要
目的通过检测体外授精-胚胎移植（IVF-ET）受孕与自然受孕胎盘组织中胰岛素样生长因子-Ⅰ、Ⅱ（IGF-Ⅰ、Ⅱ）的表达，了解IVF-ET受孕对IGF-Ⅰ、Ⅱ表达的影响。方法选择2007年1月至2009年1月，不孕因素均为输卵管因素，辅助生育技术方法为IVF-ET的49例产妇为研究组，选择同期49例健康的自然受孕孕妇为对照组。采用RT-PCR技术检测两组胎盘组织中IGF-ⅠmR-NA、IGF-ⅡmRNA表达水平，免疫组化技术检测两者胎盘组织中IGF-Ⅰ、IGF-Ⅱ蛋白含量。结果研究组胎盘中IGF-Ⅰ mRNA、IGF-ⅡmRNA相对表达量依次为0．30±0．13、0．28±0．04；对照组胎盘中IGF-ⅠmRNA、IGF-ⅡmRNA表达量为0．65±0．10、0．91±0．26，研究组IGF-Ⅰ、IGF-Ⅱ平均光密度为0．26±0．04、0．29±0．05；对照组IGF-Ⅰ、IGF-Ⅱ平均光密度为0．32±0．07、0．34±0．04。研究组胎盘中IGF-ⅠmRNA、IGF-ⅡmRNA和蛋白表达水平均低于对照组，差异有统计学意义（P〈0．05）。研究组低出生体重儿发生率8．16％，对照组低出生体重儿发生率2．04％，差异无统计学意义（P〉0．05）。结论体外授精一胚胎移植孕妇胎盘中IGF-Ⅰ及IGF-Ⅱ表达水平低于自然受孕孕妇，但两组产妇新生儿出生体重相近，且低出生体重儿比例相当，提示IGF-Ⅰ、Ⅱ有促进胎儿的生长发育的作用，对胎儿的生长发育影响不大。
Objective To study the expression changes of placental insulin-like growth factor I and Ⅱ （IGF-Ⅰ and IGF-Ⅱ） between pregnancies following the in vitro fertilization and embryo transfer （ IVF - ET） and those conceived spontaneously. Methods The placenta were collected from 49 cases of pregnancies after IVF - ET（ case group） and 49 cases of pregnancies who were normal （control group）. The expressions of IGF-Ⅰ and IGF-1I mRNA in placenta were detected by reverse transcription- polymeras chain reaction （RT-PCR）. The levels of IGF-Ⅰ and IGF-Ⅱ protein were determined by immunohistochemistry. Results The mRNA levels of the placental IGF-Ⅰ and IGF Ⅱ were 0. 30 ±0. 13 and 0. 28 _＋0. 04 in the IVF-ET group, and 0. 65 ±0. 10 and 0. 91 ＋0. 26 in the control group. The protein levels of the placental IGF-Ⅰ and IGF-Ⅱ were 0. 26 ±0. 04 and O. 29 ± 0. 05 in the IVF-ET group, and O. 32 ± 0. 07 and O. 34 ± 0. 04 in the control group. The mRNA and protein expressions of IGF-Ⅰ and IGF-Ⅱ in human placenta were significantly decreased in IVF-ET group compared to control group （ P 〈 0. 05 ）. The incidence rate of low birth weight infant in IVF-ET group was significantly higher than control（ P 〉0. 05）. Conclusions Expressions of placental 1GF-Ⅰ and IGF-Ⅱ in the IVF-ET group did not affect fetal growth and development.
出处
《中国医师杂志》
CAS
2012年第10期1335-1337,共3页
Journal of Chinese Physician
基金
国家973公关项目子课题项目（2006CB944005）
江苏省常州市科技局项目（cs2008223）
关键词
胚胎移植
受精
体外
胎盘
胰岛素样生长因子L
分析
胰岛素样生长因子Ⅱ
分析
Embryo transfer
Fertilization in vitro
Placenta
Insulin-like growth factor I/analy-sis
Insulin-like growth factor II/analysis
