摘要
目的:通过检测在风寒、寒湿和湿热环境下小鼠脾淋巴细胞的增殖能力,探讨不同外邪因素对小鼠免疫功能的影响。方法:选择刀豆蛋白(ConA)诱导脾淋巴细胞增殖,采用MTT法测定小鼠脾淋巴细胞增殖能力。结果:①各外邪因素处理组小鼠脾淋巴细胞的增殖能力皆明显低于正常组(P<0.01)。②各外邪低免组与低免组相比,风寒低免组、寒湿低免组小鼠脾淋巴细胞的增殖能力明显降低(P<0.01);湿热低免组小鼠脾淋巴细胞增殖能力也有降低,但无统计学差异。③风寒低免组与风寒组相比、寒湿低免组与寒湿组相比、湿热低免组与湿热组相比,小鼠脾淋巴细胞的增殖能力均明显降低(P<0.01)。结论:与正常组相比,各外邪因素处理组小鼠脾淋巴细胞的增殖能力都发生降低,低免模型组小鼠的淋巴细胞增殖能力下降程度尤其显著。实验表明风寒、寒湿、湿热3种外邪因素可使小鼠免疫功能降低,易导致疾病的发生。
Objective： To detect the proliferation capacity of splenic lymphocytes of mice in the cold,cold dampness and hot and humid environment,and discusses the effect of different factors of attacks on the immune functions of mice.Methods： We choose knife bean protein（ConA） induced spleen lymphocyte proliferation,use the MTT method to determine the spleen lymphatic proliferation capacity in mice.Results： ①The mice splenic lymphocytes proliferation capacity of all the attack factors processing groups were significantly lower than the normal group（P0.01）.②All the attack factors processing low immune functions groups compared with low immune function group,the splenic lymphocytes proliferation capacity of wind-cold,cold-dampness low immune function groups reduced significantly（P0.01）;the splenic lymphocytes proliferation capacity of dampness-heat low immune function groups decreasd,but this was not statistically significant.③The wind-cold low immune function group compared with the windcold group,the cold-dampness low immune function group compared with the cold-dampness group,the dampness-heat low immune function group compared with the dampness-heat group,all the mice splenic lymphocytes proliferation capacity significantly reduced（P0.01）.Conclusion： Compared with the normal group,the mice splenic lymphocytes proliferation capacity of all the attack factor processing groups reduced,the mice splenic lymphocytes proliferation capacity of low immune modle groups especially signigicant.The study shows that the three attacks factors of wind-cold,cold-dampness,dampness-heat can make the immune function of mice reduce and cause the happen of the disease easily.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2012年第11期2839-2842,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然基金资助项目(No.30873211)~~
关键词
外邪因素
免疫功能低下
淋巴细胞增殖能力
MTT法
Attacks factors
Low immune function
Lymphocyte proliferation capacity
MTT method
