摘要
目的:研究中药活性成分黄芩苷,川芎对中波紫外线(UVB)辐射损伤成纤维细胞保护作用的影响及相关作用机制。方法:采用0mJ/cm2,30mJ/cm2,60mJ/cm2,90mJ/cm2剂量UVB照射培养的原代人皮肤成纤维细胞,同时加入黄芩苷和川芎干预处理,观察细胞损伤的形态学变化,以MTT法检测细胞活性,以酶联免疫吸附实验(ELISA)检测IL-10,IL-12和TNF-α的分泌量。结果:UVB照射后,细胞损伤程度与照射剂量呈正相关,增殖活性下降;此外,UVB照射后IL-10等细胞因子分泌增加,而加入所试中药干预可明显抑制细胞损伤和相关细胞因子分泌。结论:黄芩苷、川芎有一定的光保护作用,抑制炎症细胞因子IL-10,IL-12,TNF-α的分泌可能是其减轻UVB光损伤的机制之一。
Objective To investigate damage effects of ultraviolet irradiation on human fibroblasts and to evaluate photo-protective efficiency of Traditional Chinese Medicines (Baicalin and Szechwan Lovge Rhizome) on fibroblasts damaged by UVB irradiation. Methods Subconfluent fibroblasts were sham or UVB irradiated and treated with above TCM agents. The damage degree of fibroblasts was observed by a light microscop. Cell growth was recorded by cell count and cellular activity was detected by MTT method. The secretion amount of IL-10, IL-12 and TNF-α was measured by ELISA. Results The damage of fibroblasts was depended on the irradiated dosages and cellular activity was reduced. The intervention of the above drugs may increase the cellular activity. As to cytokine secretion, baicalin and szechwan lovge rhizome could reduce the secretion of IL-10,1L-12 and TNF-α induced by UVB irradiation. Conclusion The injury effect of UVB irradiation on cultured fibroblasts is dose-dependent and the tested drugs have photo-protective potency. Inhibition of cytokine secretion may be one of the mechanisms related to reducing the damage effect of UVB irradiation.
出处
《中国美容医学》
CAS
2007年第7期977-980,共4页
Chinese Journal of Aesthetic Medicine