摘要
目的:观察慢性光化性皮炎(CAD)患者外周血T细胞亚群及血清白介素-17(IL-17)、白介素-22(IL-22)、sFas、sFasL的变化及意义。方法:选取我院确诊为慢性光化性皮炎患者48例(观察组),取患者晨起空腹静脉血,采用流式细胞术和ABC-ELISA双抗体夹心法检测CAD患者外周血T淋巴细胞亚群、Thl7相关因子、IL-17、IL-22和外周血凋亡蛋白sFas、sFasL的表达,并设同期来院体检正常人30例为对照组,进行对比分析。结果:观察组患者CD3+CD4+、CD3+CD4+/CD3+CD8+显著低于对照组(P<0.05),CD3+CD8+显著高于对照组(P<0.05);观察组患者血清IL-22、sFas、sFasl显著高于对照组(P<0.05);两组CD3+、IL-17比较无统计学差异(P>0.05)。结论:CAD患者存在不同程度的免疫系统功能紊乱,T淋巴细胞参与其迟发型超敏反应;IL-22在CAD发病过程中起促进作用,外周细胞凋亡蛋白表达异常亦在其发病中起一定的作用。
Objective：To observe the change and significance of peripheral blood T cell subset and serum IL-17,IL-22,sFas and sFasL in the patients with chronic actinic dermatitis（CAD）.Methods：A total of 48 patients diagnosed with CAD were enrolled in our study and served as the observation group.Morning fasting venous blood was collected.The flow cytometry and ABC-ELISA double antibody sandwich method were used to detect the peripheral blood T cell subset,Th17,IL-17,IL-22,sFas and sFasL levels in the patients with CAD.And 30 cases with the normal physical examinations at the same stage served as the control group.And the data in the two groups were compared and analyzed.Results：CD3＋CD4＋level and CD3＋CD4＋/CD3＋CD8＋ratio in the observation group were significantly lower than those in the control group（P〈0.05）,and the CD3＋CD8＋level was significantly higher than that in the control group（P〈0.05）.The serum IL-22,sFas and sFasL levels in the observation group were significantly higher than those in the control group（P〈0.05）.There was no significant difference in CD3＋and IL-17 levels in the two groups（P〉0.05）.Conclusions：There is a dysfunction of immune system in the CAD patients in a different degree.It showed that T lymphocytes participate in the delayed type hypersensitivity,IL-22 plays a driving role in the CAD disease course,and the abnormal peripheral cell apoptosis protein expression plays a certain role in the disease course.
出处
《海南医学院学报》
CAS
2015年第1期71-72,76,共3页
Journal of Hainan Medical University
基金
河北省科学技术成果项目(20113459)~~
