摘要
采用PHA体内直接注射法制备了黄姑鱼Nibea albiflora和日本黄姑鱼N. japonica肾组织的染色体制片,并对两种鱼的核型进行了比较分析。结果表明,黄姑鱼的核型公式为:2n=48t,NF=48;日本黄姑鱼的核型公式为:2n=48t,NF=48。黄姑鱼染色体相对长度最长为5.94±0.33,最短为3.06±0.20;日本黄姑鱼染色体相对长度最长为5.68±0.22,最短为2.66±0.14。收集文献报道的石首鱼科鱼类的核型研究资料,包括双棘黄姑鱼N.diacanthus、鱼免状黄姑鱼N.miichthioides、大黄鱼Pseudosciaena crocea的核型,应用核型进化距离聚类分析方法研究了这5种石首鱼科鱼类的亲缘关系。结果表明,黄姑鱼和双棘黄姑鱼以及鱼免状黄姑鱼和日本黄姑鱼的亲缘关系较近,大黄鱼与鱼免状黄姑鱼的亲缘关系最远,核型进化距离聚类分析在一定程度上反映了这5种鱼的亲缘关系和进化历程。
The metaphase chromosomes of Nibea albiflora （Richardson）and Nibea japonica were obtained from kidney tissue by the method of PHA and colchicines injection, and the karyotype of these two fishes were analyzed. The results showed that both fishes had 48 telo centric chromosomes in the diploid, and the karyotype formula was 2n= 48t, NF= 48. The rel ative length of chromosomes ranged from 3.06±0.20 to 5.94±0.33 in N. albiflora, and from 2.66±0.14 to 5.68±0.22 in N. japonica. Karyotype data of Sciaenidae species in previous re- ports were also collected including N. diacanthus, N. rniichthioide and Pseudosciaena crocea.The clustering analysis was carried out for karyotype evolutionary distance among N. albiflo- ra, N. japonica, N. diacanthus, N. miichthioide,and P. crocea. The results showed that the rela- tionship between N. albi flora and N. diacanthus, and between N. japonica and N. miichthioide, were relatively close based on karyotype evolutionary distance, and the largest karyotype evolutionary distance （0. 064 8） were found between N. miichthioide and P. crocea. Results from the clustering analysis were consistent with the phylogeny based on the morphology of these species.
出处
《渔业科学进展》
CSCD
北大核心
2012年第5期8-14,共7页
Progress in Fishery Sciences
基金
浙江省科技计划项目(2009C12081、2010F20006、2012C12907-8)
浙江省海洋与渔业局海域海岛项目(浙海渔计[2010]205)
国家863计划(2012AA10A413-5)共同资助
关键词
黄姑鱼
日本黄姑鱼
染色体
核型进化距离
聚类分析
Nibea albi flora Nibea japonica Karyotype evolutionary distance Chromosome Cluster analysis
