摘要
目的 掌握多孔涂层假体标本制作方法 ,开展对植入体内多孔涂层假体涂层界面内 (porous -coatedprosthesisinterface)骨长入观察。 方法 收集植入体内多孔涂层假体因其它原因而被翻修者 1 0例。用纯丙酮液浸泡 ,间隔更换丙酮液 ,约 2周使标本骨组织完全脱水干燥。以国产钻石刀粗切后低温塑料包埋 ,再用进口钻石切片机切片 ,甲苯胺蓝染色后光镜观察 ,表面喷金后电镜观察。结果 利用国产钻石切割刀先进行标本的粗制作 ,再用进口切片机切片克服了由于国内设备条件限制尚难以开展对植入体内多孔涂层假体表面骨长入研究的困境。对固定牢固的翻修假体观察到骨长入程度不尽相同 ,股骨柄侧多于髋臼侧 ,微孔涂层界面多于珍珠涂层界面。结论 运用标本制作方法能够满足对植入体内多孔涂层假体骨长入观察 ;骨长入证实及患者的良好临床效果说明在国人中应用多孔涂层假体通过骨长入从而固定假体的可靠性 ,为今后深入的研究建立了方法学。
Objectives exploring the methods of the specimen production of retrieved porous-coated prosthesis,beginning the observation of bone ingrowth into the porous-coated prosthesis interface.Methods Collecting 10 examples which were retrieved because of no prosthesis looseness.The prosthesis were immersed in the acetone for about two weeks,the acetone bath changed daily,untill dehydration was completed.the implants were resectioned into course specimens at first,then were embeded by methacrylate.Subsequently the coarse specimens were cut into thin slice with imported leize dimmond knife,the specimens were stained with tolnidine blue and were sprayed with gold respectively.which were observed with light microcopy and electron imaging.Results by mean of the described methods,We can produce the thin slice specimen to observe the bone ingrowth into the porous coating.according to our discovery,the bone ingrowth is more within the microporous coating interface than within the macro-porous coating interface.Conclusions The methods of specimen production that we utilize can satify for the observation of bone ingrowth.the confirm of bone ingrowth and the good clinical effect of patients explain the reliablity of applying porous-coated prostheses in china.the methods of specimen production was established for the research. [
出处
《宁夏医学杂志》
CAS
2000年第9期516-517,共2页
Ningxia Medical Journal
