摘要
目的探讨临床怀本中凝固酶阴性葡萄球菌（coagulasenegativeStaphylococci，CNS）生物膜形成的状况，及其对细菌耐药的影响和多重耐药的关系，提高临床对CNS感染的重视，并帮助临床根据药敏试验结果选用抗菌药物。方法采用微孔板法制备生物膜，结晶紫染色，酶标仪比色检测42株CNS的生物膜形成情况；对临床标本分离的CNS进行鉴定，并采崩纸片法进行药敏试验；所有数据采用SPSS17．0统计软件进行统计学分析。结果比色法检测CNS的生物膜阳性率为76．19％（32／42）。药敏试验结果显示生物膜阳性的CNS耐药情况较严重，尤其是对青霉素G和红霉素，耐药率分别为96．88％和78．13％，对复办新诺明、克林霉素、四环素、环丙沙星耐药率亦超过50．00％，且呈多重耐药；生物膜阴性的CNS耐药情况较轻，仅对青霉素G和红霉素耐药情况较严重，耐药率分别为60．00％和140．00％；尚未发现对万古霉素耐药的CNS菌株。生物膜阳性CNS菌株对青霉素G、红霉素、克林霉索和复方新诺明的耐药率均显著高于生物膜阴性CNS菌株，差异均有统计学意义（P均〈0．05）。CNS生物膜生成多少与多重耐药的严重情况呈正相关性（r=0．975，P〈0．01）。结论临床标本分离出的CNS绝大部分能产生生物膜，对常见抗菌药物的耐约情况严重，且生物膜形成多少与多重耐药情况呈正相关关系。
Objective To study the formation of bacterial biofilm（BBF） ofcoagulase negative Staphy- lococci （CNS） and the influence on antibiotics resistance as well as the multiple antibiotics resistance by it. So as to draw doctors attention of this situation and to help them choosing antibiotics based on antibiotics suscep- tibility testing results correctly. Methods BBF of 42 strains CNS was formed by mieroplate method, detected by crystal violet staining and got colorimetry assay. Identified CNS and detected antibiotics resistance. Data were analyzed by SPSS 17.0 statistical software. Results BBF was pusitive by 76.19%（32/42） to CNS by col- orimetry assay. Antibiotics sensitivity testing results shown that CNS with BBF had a high antibiotics resistance to penicillin G, erythromycin, resistance rate were 96.88% ,78.13%. And to cotrimoxazole, elindamycin, te- tracyeline and ciprofloxacin, the resistance rates were all above 50.00%. The drug resistant situation of CNS withnul BFF was lighter, only to penicillin G and erythromycin＇s drug resistant rates were higher （60.00%, 40.00% ）. There was no CNS strain was resistant to vancomycin. The drug resistance rates of CNS with BBf strains to penicillin G, erythromycin, clindamycin and cntrimoxazole were higher than CNS without BBF strains, the difterences had statistical significance （Pall〈 0.05）. Also thew were muhiple antibiotics resistance situations existed and positive correlation with the formatiun of BBF（r= 0.975,P〈 0.01 ）. Conclusion Certain proportion of CNS elinical isolated could form BBF. CNS had strong muhiple antibiotics resistance. And there was a positive c.orrelation between the formation of BBF of CNS and the multiple antibiotics resistance situation.
出处
《实用检验医师杂志》
2012年第3期145-148,共4页
Chinese Journal of Clinical Pathologist
关键词
凝固酶阴性葡萄球菌
细菌生物膜
耐药性
多重耐药
纸片法
Coagulase negative Staphylococci
Bacterial biofilm
Antibiotics resistance
Multiple an-tibiotics resistance
Disc diffusion method
