摘要
目的研究68株临床分离表皮葡萄球菌大环内酯耐药性与生物膜形成能力、icaA基因型之间的关系,初步预测采用大环内酯抗菌药物预防表皮葡萄球菌生物膜感染的有效性。方法以琼脂平板稀释法测定菌株的最小抑菌浓度,采用微孔测定法考察临床分离表皮葡萄球菌生物膜形成能力,通过PCR法测定icaA基因型,探讨三者之间的关系。结果临床分离表皮葡萄球菌对大环内酯耐药程度较高(88.2%),且耐药菌生物膜形成能力较敏感菌显著增强(P<0.05),耐药菌与敏感菌的icaA阳性比率没有统计学差异(P>0.05)。结论红霉素、阿奇霉素、克拉霉素等常用的大环内酯类药物可能难以有效地预防表皮葡萄球菌生物膜感染。
Objective To study the macrolide-resistance,ability of biofilm formation and icaA-genetypes of 68 stains of clinically isolated Staphylococcus epidermidis（S.epidermidis） so as to explore the efficacy of macrolide to prevent biofilm-associated infections caused by S.epidermidis.Methods Minimum inhibitory concentration（MIC） were detected by agar-plate dilution method,and microtiter-plate assay was used to investigate the ability of biofilm formation,and icaA genetype was identified by PCR.Results High macrolide-resistant rate （88. 2% ） was showed for clinical isolated S. epidermidis, and biofilms for macrolide-resistant strains were significantly stronger than that of macrolide-sensitive strains, but there was no dependability found between these two for icaA positive rate. Conclusion Frequently-used macrolide such as erythromycin, azithromycin, and clarithromycin may incompetence to prevent biofilm-associated infections caused by S. epidermidis.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2009年第22期2252-2254,共3页
Journal of Third Military Medical University
基金
国家自然科学基金(30572366)~~
