摘要
目的利用MR灌注加权成像(perfusion weighted imaging,PWI)研究兔急性重度失血性休克模型不同时间点脑部微循环变化特点。方法 20只新西兰大白兔,按全身血量(70 ml/kg体重)的40%～50%放血,使平均动脉压维持在30 mmHg(1 mmHg=0.133 kPa),建立失血性休克模型。随机分为休克组(n=10)和复苏组(n=10)。复苏组于休克90 min后给予自身血液回输。记录两组不同时间点的平均动脉压、心率、体温、呼吸频率,测定动脉血气[pH值,血红蛋白(Hb),乳酸(Lac)等],并行PWI。采用重复测量数据的方差分析。结果平均动脉压、心率、体温、血pH值、Hb、Lac、相对脑血容量(rCBV)及相对脑血流量(rCBF)在复苏组与休克组之间差异有统计学意义(P<0.05)。休克组在休克时rCBV较基线显著升高(P<0.05),休克90 min rCBF较基线显著升高(P<0.01),休克150 min时平均通过时间(MTT)、达峰时间(TTP)、到达时间(T0)较基线显著升高(P<0.05)。结论T2*WI PWI能够有效观察急性重度失血性休克及复苏后脑微循环变化。
Objective The aim of this study was to investigate using PWI whether rabbits have the timing and degree of microcirculation changes in the brain of rabbits during severe hemorrhagic shock and resuscitation in real time.Methods Twenty New Zealand rabbit were randomized to undergo hemorrhagic shock,induced by a 40%-50% blood volume bloodletting,and maintained MAP at 30 mmHg,for 90 min with（n = 10） or without（n = 10） subsequent resuscitation.Resuscitated animals received their own shed blood.The blood pressure,heart rate,temperature,respiratory rate,and arterial blood gas content were recorded during severe hemorrhagic shock and resuscitation in real time.At the same time,the two groups underwent perfusion-weighted imaging（PWI）.The analysis of variance of repeated data was made.Independent sample t test between shock group and resuscitation was made.Results MAP、 HR、T、PH、Hb、Lac、relative cerebral blood volume（rCBV）、relative cerebral blood flow（rCBF） showed significant differences in the shock group and resuscitation group（P〈0.05）.rCBV was significantly increased in shock（P〈0.05）.rCBF were significant increased at 90 min time points in shock（P〈0.01）.MTT、TTP 、T0 were significantly increased at 150 min time points comparing with the baseline points in shock（P〈0.05）.Conclusion T2＊ PWI can effectively observe the cerebral microcirculation changes during acute severe hemorrhagic shock and resuscitation.
出处
《临床放射学杂志》
CSCD
北大核心
2012年第10期1480-1485,共6页
Journal of Clinical Radiology
关键词
失血性休克
脑微循环
磁共振成像
Hemorrhagic shock Cerebral microcirculation Magnetic resonance imaging
