摘要
目的:建立HPLC法测定七味胃痛胶囊中胡椒碱的含量,以有效控制该制剂胡椒碱的质量。方法:以InertsitC18柱(4.6mm×250mm,5um)为色谱柱;以甲醇-水(77∶23)为流动相;检测波长343nm;柱温30℃;流速1.0ml·min-1。结果:胡椒碱进样量在4.275～85.52μg·ml-1范围内线性关系良好(r=0.9998),平均回收率为98.4%,RSD为0.6%(n=5),样品在24h内稳定。结论:本文建立的含量测定方法简捷快速,结果准确可靠,可用于七味胃痛胶囊的质量控制。
Objective： To establish a method for determination of seven kinds of HPLC Weitong capsule of pipefine content, in older to effectively control the preparation of piperine quality. Methods： the Inertsit C18 column （4.6mm × 250mm,5um） is a chromatographic column; methanol - water（77：23 ）as mobile; detection wavelength：343nm ; column temperature30 degrees Celsius; velocity 1.0ml· min^-1. Results：Pepper alkali amount of sample in the 4.275N85.52μg· ml^-1 within a good linear relationship （1=0.9998）, the average recovery was 98.4%,RSD was 0.6%（n=5）,the sample in the 24h stability.Conclusion： the established method of determination the content of simple and rapid, accurate and reliable resuhs, can be used for the quality control of seven flavor capsule for treating stomachache.
出处
《北方药学》
2012年第11期1-1,共1页
Journal of North Pharmacy
关键词
七味胃痛胶囊
胡椒碱
HPLC
Seven taste Weitong capsule Pepper alkali HPLC
